2012 Fiscal Year Final Research Report
Development of novel fluorescence probes and imaging technologies for elucidating the mechanism of cellular signaling and responses induced by ROS
| Project Area | Signaling functions of reactive oxygen species |
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| Project/Area Number |
20117003
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo |
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Principal Investigator |
URANO Yasuteru 東京大学, 大学院・医学系研究科, 教授 (20292956)
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| Project Period (FY) |
2008 – 2012
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| Keywords | 蛍光プローブ / 活性酸素種 / シグナル / 親電子性活性種 / イメージング |
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| Research Abstract |
For elucidating the functions of ROS and RNS as intracellular signaling molecules, we aimed at developing novel optical imaging probes which afford their live-imaging in living biological samples. We have succeeded in developing fluorescence probes for hydrogen peroxide, acrolein, and the enzymatic activity of glutathione S-transferase, those for highly ROS and NO which has much desired property of intracellular retention for the long-period observation, that for hypochlorite with NIR emitting capability, and luminescence probes for detecting hROS. We also have succeeded to obtain various striking images of each ROS and RNS in living cells and animals.
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[Journal Article] Rapid Cancer Detection by Topically Spraying a Gamma-glutamyltranspeptidase-activated Fluorescent Probe2011
Author(s)
Urano Y, Sakabe M, Nobuyuki Kosaka, Ogawa M, Mitsunaga M, Asanuma D, Kamiya M, Young MR, Nagano T, Choyke PL, Kobayashi H
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Journal Title
Sci Transl Med
Volume: 3
Pages: 110ra119
DOI
Peer Reviewed
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[Journal Article] Selective molecular imaging of viable cancer cells with pH-activatable fluorescence probes2009
Author(s)
Urano Y, Asanuma D, Hama Y, Koyama Y, Barrett T, Kamiya M, Nagano T, Watanabe T, Hasegawa A, Choyke PL, Kobayashi H
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Journal Title
Nat. Med
Volume: 15
Pages: 104-109
DOI
Peer Reviewed
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