ROS sensor-effector regulation via Cys modifications

2010 Fiscal Year Self-evaluation Report

• Project Area: Signaling functions of reactive oxygen species
• Project/Area Number: 20117013
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyushu University
• Principal Investigator: NISHIDA Motohiro Kyushu University, 薬学研究院, 准教授 (90342641)
• Project Period (FY): 2008 – 2012
• Keywords: 活性酸素 / 受容体 / G蛋白質 / システイン酸化 / 心臓

Research Abstract

様々なリガンド刺激で誘発される生理応答(例えば受容体の発現調節)におけるシグナル蛋白質の酸化的機能修飾の役割を明らかにする。具体的には、細胞外ヌクレオチドや細菌毒素が細胞膜上の受容体を刺激することで生成される活性酸素種(ROS)や一酸化窒素(NO)によって局所的に機能修飾を受ける細胞質タンパク(活性酸素受容体)を特定し,ROS/NO生成酵素との相互作用を示す。また、特定した活性酸素受容体の心血管病における役割を明らかにする。これにより、ROS/NOシグナルソーム形成の意義を明らかにする。

Research Products

• [Journal Article] Heterologous down-regulation of angiotensin typel receptors by purinergic P2Y_2 receptor stimulation through S-nitrosylation of NF-κB (2011)
  • Author(s): Nishida M, Ogushi M, Suda R, Toyotaka M, Saiki S, Kitajima N, Nakaya M, Kim K-M, Ide T, Sato Y, Inoue K , Kurose H.
  • Journal Title: Proc.Natl.Acad. Sci.USA 108 Pages: 6662-6627
  • Peer Reviewed
• [Journal Article] Regulation of angiotensin II receptor signaling by cysteine modification of NF-κB (2011)
  • Author(s): Nishida M, Kitajima N, Saiki S, Nakaya M., Kurose H.
  • Journal Title: Nitric Oxide in press
  • Peer Reviewed
• [Journal Article] Pertussis toxin upregulates angiotensin typel receptors through TLR4-mediated Rae activation (2010)
  • Author(s): Nishida M, Suda R, Tanabe S, Onohara N, Nakaya M, Kanaho Y, Sumimoto H, Sato Y , Kurose H.
  • Journal Title: J.Biol. Chem 285 Pages: 15268-15277
  • Peer Reviewed
• [Journal Article] Phosphorylation of TRPC6 channels at Thr^<69> is required for anti-hypertrophic effects of phosphodiesterase 5 inhibition (2010)
  • Author(s): Nishida M, Watanabe K, Sato Y, Nakaya M, Kitajima K, Ide T, Inoue R, Kurose H.
  • Journal Title: J.Biol. Chem 285 Pages: 13244-13253
  • Peer Reviewed
• [Journal Article] Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound (2009)
  • Author(s): Kiyonaka S, Kato K, Nishida M, Mio K, Numaga T, Sawaguchi Y, Yoshida T, Wakamori M, Mori E, Numata T, Ishii M, Takemoto H, Ojida A, Watanabe K, Uemura A, Kurose H, Morii T, Kobayashi T, Sato Y, Sato C, Hamachi I, Mori Y
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 5400-5405
  • Peer Reviewed
• [Journal Article] P2Y_6 Receptor-Gα_<12/13>3 Signaling in Cardiomyocytes Triggers Pressure Overload-induced Cardiac Fibrosis (2008)
  • Author(s): Nishida M, Sato Y, Uemura A, Narita Y, Tozaki-Saitoh H, Nakaya M, Ide T, Suzuki K, Inoue K, Nagao T, Kurose H.
  • Journal Title: EMBO J 27 Pages: 3104-3115
  • Peer Reviewed
• [Presentation] Down-regulation of angiotensin receptors by S-nitrosylation of NF-KB.Society for Free Radical Research (SFRR) International (2009)
  • Author(s): Nishida M
  • Organizer: Free Radical School in Japan
  • Place of Presentation: Joetsu Kokusai Ski Resort
  • Year and Date: 2009-09-03
• [Book] Heterotrimeric G proteins in heart failure Heart Failure : Symptoms(Causes , Treatment Options)(NOVA Publishers (New York)) (2010)
  • Author(s): Nishida M, Ohba M, Nakaya M , Kurose H
  • Total Pages: 51-72
  • Publisher: Edited by Wright MS
• [Remarks] ホームページ
  • URL: http://chudoku.phar.kyushu-u.ac.jp/
• [Remarks] 研究成果の報道
  • URL: http://www.kyushu-u.ac.jp/pressrelease/index.php