ATP hydrolysis and the energy of ATP driven proteins and correlationanalysis of the hydration state

2010 Fiscal Year Self-evaluation Report

• Project Area: Water plays a key role in ATP hydrolysis and ATP-driven functions of proteins
• Project/Area Number: 20118008
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Tohoku University
• Principal Investigator: SUZUKI Makoto Tohoku University, 大学院・工学研究科, 教授 (60282109)
• Project Period (FY): 2008 – 2012
• Keywords: ATPエネルギー / 水和 / 水のエントロピー / 加水分解自由エネルギー

Research Abstract

ATPの加水分解エネルギーの物理的実体はいったい何なのか?この究極的問題を解明するため、ATP分解にともなう水の動的状態の変化を調べる。本研究では、「構造」、「ダイナミクス」、「エネルギー論」を統合するエネルギー変換理論の確立を目指して、ハイパーモバイル水(HMW)を軸にした水和の分子論を展開する。具体的には、誘電緩和法によるATPおよびその加水分解産物ADPなどの水和特性を測定し、HMWを含め水和各成分の量的比率を求める。アクチンおよびミオシンの水和状態の特性を誘電緩和法による回転緩和情報とパルス磁場勾配スピンエコー(PFG-SE) NMRによる並進拡散係数から明らかにし、アクトミオシンによるATP加水分解過程の水和特性変化を評価する。また、ATP加水分解過程の反応熱を実験的に再検討する。これらの結果をもとに、溶液化学・統計熱力学の理論家と共同して自由エネルギー変化の内部構成(内部エネルギー項、エントロピー項)を解析し、ATP加水分解エネルギーの物理的描像を構築する。

Research Products

• [Journal Article] Hyper-mobility of water around actin filaments revealed using pulse-field gradient spin-echo 1H-NMR and fluorescence spectroscopy (2011)
  • Author(s): T.Wazawa, T.Sagawa, T.Ogawa, N.Morimoto, M.Suzuki
  • Journal Title: Biochem.Biophys.Res.Comm. 404 Pages: 985-990
  • Peer Reviewed
• [Journal Article] Hydration properties of adenosine phosphate series as studied by microwave dielectric spectroscopy (2010)
  • Author(s): G.Mogami, T.Wazawa, N.Morimoto, T.Kodama, M.Suzuki
  • Journal Title: Biophys.Chem. 154 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Entropic Potential Field Formed for a Linear-Motor Protein near a Filament : Statistical-Mechanical Analyses Using Simple Models (2010)
  • Author(s): K.Amano, T.Yoshidome, M.Iwaki, M.Suzuki, M.Kinoshita
  • Journal Title: J.Chem.Phys. 133 Pages: 045103
  • Peer Reviewed
• [Journal Article] Hydration analysis of Pseudomonas aeruginosa cytochrome c551 upon acid unfolding (2010)
  • Author(s): T.Wazawa, T.Miyazaki, Y.Sambongi, M.Suzuki
  • Journal Title: Biophys.Chem. 151 Pages: 160-169
  • Peer Reviewed
• [Journal Article] A Statistical-Mechanical Analysis on the Hyper-Mobile Water around a Large Solute with High Surface Charge Density (2009)
  • Author(s): M.Kinoshita, M.Suzuki
  • Journal Title: J.Chem.Phys. 130 Pages: 014707(1-11)
  • Peer Reviewed
• [Journal Article] Measurement of the dielectric relaxation property of water-ion loose complex in aqueous solutions of salt at low concentrations (2008)
  • Author(s): T.Miyazaki, T.Wazawa, G.Mogami, T.Kodama, M.Suzuki
  • Journal Title: J.Phys.Chem.A 112 Pages: 10801-10806
  • Peer Reviewed
• [Presentation] Hydration measurement of salt ions, polyelectrolytes, and proteins in dilute solutions by high resolution dielectric relaxation spectroscopy (2010)
  • Author(s): Makoto Suzuki
  • Organizer: TRILATERAL SCIENTIFIC SEMINER ON "Solvation in Complex Liquids : Bridging Length Scales by Theory and Experiment
  • Place of Presentation: Max Planck Institute, Leipzig, Germany 招待講演
  • Year and Date: 20100623-20100625
• [Presentation] Dielectric relaxation spectroscopy analysis of hydration of polyelectrolytes and proteins (2010)
  • Author(s): Makoto Suzuki
  • Organizer: WORKSHOP "SOLVATION OF BIOACTIVE COMPOUNDS"
  • Place of Presentation: Max Planck Institute, Leipzig, Germany 招待講演
  • Year and Date: 20100107-20100109