2012 Fiscal Year Final Research Report
Hydration and Energetic Study on ATP Hydrolysis and ATP-driven Proteins
| Project Area | Water plays a key role in ATP hydrolysis and ATP-driven functions of proteins |
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| Project/Area Number |
20118008
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Complex systems
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| Research Institution | Tohoku University |
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Principal Investigator |
SUZUKI Makoto 東北大学, 大学院・工学研究科, 教授 (60282109)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Nobuyuki 東北大学, 大学院・工学研究科, 准教授 (00313263)
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| Project Period (FY) |
2008 – 2012
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| Keywords | ATPエネルギー / タンパク質の水和 / ATP加水分解反応 / 誘電緩和分光 / 水和 / 溶解和 / リン酸の水和 / ハイパーモバイル水 |
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| Research Abstract |
Hypermobile water(HMW)at phosphoryl moiety and also constrained water(CW)at adenosine portion were detected in the hydration layer of ATP by using high-resolution dielectric relaxation spectroscopy(DRS). HMW has been theoretically corroborated as higher rotational entropy density of water than that of bulk around charged particle(collaboration with Kinoshita)and faster relaxation time region of water around charged ions by MD.(collaboration with Akiyama group and Matubayasi) There has been observed CW and HMW in the hydration layer of actin. When actin monomers polymerize to become a filament at the high temperature, remarkable increase in HMW was observed, which indicated the strong relationship between this endothermic reaction and HMW formation.
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