Single molecule analysis of the mechano-chemical conversion mechanism for ATP-driven motor proteins.

2010 Fiscal Year Self-evaluation Report

• Project Area: Water plays a key role in ATP hydrolysis and ATP-driven functions of proteins
• Project/Area Number: 20118009
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Institute of Physical and Chemical Research (2012-2013); Osaka University (2008-2011)
• Principal Investigator: IWAKI Mitsuhiro Osaka University, 医学系研究科, 助教 (30432503)
• Project Period (FY): 2008-11-13 – 2013-03-31
• Keywords: モーター蛋白質 / ミオシン / 1分子計測 / 1分子操作 / レーザートラップ / 化学力学変換

Research Abstract

本研究では、ATPのエネルギーを使って駆動するアクトミオシン系のエネルギー変換の素過程を1分子レベルで解析し、周囲環境の水分子の寄与を取り入れた形でモデル化を行っている。水分子の寄与を評価するに当たって、まずは、溶媒中にショ糖などの共溶媒を添加し水和状態に摂動を加え、アクトミオシンの機能がどのように変調されるか多分子系と1分子系で観察を行う。従来の1分子計測では観察が難しい運動素過程への共溶媒の影響を調べるために新規計測系、技術の開発も並行して行う。

Research Products

• [Journal Article] Entropic potential field formed for a linear-motor protein near a filament : Statistical-mechanical analyses using simple models (2010)
  • Author(s): K.Amano, T.Yoshidome, M.Iwaki, M.Suzuki, M.Kinoshita
  • Journal Title: Journal of Chemical Physics Vol.133 Pages: 045103-1-045103-11
  • Peer Reviewed
• [Journal Article] Brownian search-and-catch mechanism for myosin-VI steps (2009)
  • Author(s): M.Iwaki, A.Iwane, T.Shimokawa, R.Cooke, T.Yanagida
  • Journal Title: Nature Chemical Biology Vol.5 Pages: 403-405
  • Peer Reviewed
• [Presentation] Brownian search-and-catch mechanism for myosin-VI steps (2011)
  • Author(s): M.Iwaki
  • Organizer: Gordon research conferences-Stochastic physics in biology
  • Place of Presentation: Ventura, California.
  • Year and Date: 20110123-20110129
• [Presentation] Observation of mechanical process for myosin-II by a dark-field microscopy combined with an optical trap (2011)
  • Author(s): M.Iwaki
  • Organizer: Biophysical Society 55^<th> Annual meeting
  • Place of Presentation: Baltimore, Maryland
  • Year and Date: 2011-03-05
• [Presentation] Effect of Cosolvent on Brownian search-and-catch mechanism for actomyosin (2010)
  • Author(s): M.Iwaki
  • Organizer: International Symposium on Hydration and ATP energy
  • Place of Presentation: 仙台
  • Year and Date: 20100308-10
• [Presentation] Brownian search-and-catchmechanism for myosin-VI steps (2010)
  • Author(s): M.Iwaki
  • Organizer: Biophysical society 54^<th> annual meeting
  • Place of Presentation: San Francisco
  • Year and Date: 20100220-20100224
• [Presentation] 共溶媒の効果:ATP駆動モーター蛋白質の1分子計測の場合 (2010)
  • Author(s): 岩城光宏
  • Organizer: 日本生物物理学会第65回年次大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2010-03-22
• [Presentation] ナノ粒子を利用したモーター蛋白質の高速1分子ナノイメージングと操作 (2009)
  • Author(s): 岩城光宏
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-21
• [Book] 生物物理(ストレインセンサーが制御するミオシンVIのブラウン運動整流機構) (2010)
  • Author(s): 岩城光宏
  • Total Pages: 88-89
  • Publisher: 日本生物物理学会
• [Book] Annual Report of Osaka University(Academic Achievement- 2009-2010) (2010)
  • Author(s): Mitsuhir Iwaki
  • Total Pages: 1
  • Publisher: 大阪大学出版会