Single Molecule analysis for the Mechano-Chemical Conversion of ATP-driven proteins

2013 Fiscal Year Final Research Report

• Project Area: Water plays a key role in ATP hydrolysis and ATP-driven functions of proteins
• Project/Area Number: 20118009
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Institute of Physical and Chemical Research (2012-2013); Osaka University (2008-2011)
• Principal Investigator: IWAKI Mitsuhiro 独立行政法人理化学研究所, 生命システム研究センター, 上級研究員 (30432503)
• Project Period (FY): 2008-11-13 – 2013-03-31
• Keywords: ATP加水分解酵素 / モーター蛋白質 / アクトミオシン / １分子計測

Research Abstract

Water is a origin of life and various biomolecules like myosin which induces muscle contraction emerge functions in water environment. It is known that Myosin converts chemical energy of ATP into mechanical work, however, the role of water in the conversion mechanism was unclear. Here, we observed elementary mechanical processes of myosin in action in the presence of osmolyte. we found entropic potential field of water molecules formed for a actomyosin interface regulates binding force between myosin and actin. Further, hydration/dehydration dynamics around actomyosin affected force generation. We suggest water explicitly play an important role in the force generation in terms of energy conversion.

Research Products

• [Journal Article] ブラウン型ナノマシンの作動原理 (2013)
  • Author(s): 岩城 光宏
  • Journal Title: 生物物理会誌 Volume: 53巻 Pages: 164-165
  • DOI: 10.2142/biophys.53.164
  • Peer Reviewed
• [Journal Article] Switching of myosin-V motion between the lever-arm swing and Brownian search-and-catch (2012)
  • Author(s): K.Fujita, M.Iwaki, A.Iwane, L.Marcucci, T.Yanagida
  • Journal Title: Nature Communications Volume: 3
  • DOI: 10.1038/ncomms1934
  • Peer Reviewed
• [Journal Article] Entropic potential field formed for a linear motor protein near a filament : Statistical mechanical analyses using simple models (2010)
  • Author(s): K.Amano, T.Yoshidome, M.Iwaki, M.Suzuki, M.Kinoshita
  • Journal Title: Journal of Chemical Physics Volume: 133 Pages: 045103
  • DOI: 10.1063/1.3462279
  • Peer Reviewed
• [Journal Article] ストレインセンサーが制御するミオシンVI のブラウン運動整流機構 (2010)
  • Author(s): 岩城光宏
  • Journal Title: 生物物理学会誌 Volume: 50巻 Pages: 88-89
  • DOI: 10.2142/biophys.50.88
  • Peer Reviewed
• [Journal Article] Brownian search-and-catch mechanism for myosin-VI steps (2009)
  • Author(s): M.Iwaki, A.Iwane, T.Shimokawa, R.Cooke, T.Yanagida
  • Journal Title: Nature Chemical Biology Volume: 5
  • DOI: 10.1038/nchembio.171
  • Peer Reviewed
• [Presentation] Designing of Self-assembled Bio molecular System and the Detection at the Single Molecule Resolution (2014)
  • Author(s): M.Iwaki, T.Yanagida, W.Shih
  • Organizer: 58^<th> Annual Meeting, Biophysical Society
  • Place of Presentation: Moscone Center, San Francisco, USA
  • Year and Date: 20140215-19
• [Presentation] Designing of Self-assembled Bio molecular System and the Detection at the Single Molecule Resolution (2013)
  • Author(s): M.Iwaki, T.Yanagida, W.Shih
  • Organizer: 第51回生物物理学会年会
  • Place of Presentation: 京都国際会館、京都
  • Year and Date: 20131028-30
• [Presentation] Multiple regulation of force generation mode for single actomyosin motor (2013)
  • Author(s): M.Iwaki
  • Organizer: 7^<th> International Conference on Engineering Complexity
  • Place of Presentation: Hotel Hohe Dune, Rostock-Warnemunde, Germany
  • Year and Date: 20130610-13
  • Invited
• [Presentation] アクトミオシンモーターの1分子計測から観たATP エネルギー変換と共溶媒の効果 (2013)
  • Author(s): 岩城 光宏
  • Organizer: 新学術領域研究公開終了シンポジウム
  • Place of Presentation: KKR 東京、東京
  • Year and Date: 2013-05-22
  • Invited
• [Presentation] Discovery of strain-sensor mechanism in motor protein and the quantification for the energy conversion (2012)
  • Author(s): M.Iwaki
  • Organizer: The 50th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: 名古屋大学、愛知
  • Year and Date: 20120922-24
  • Invited
• [Presentation] Brownian search-and-catch mechanism in motor protein and the quantification for the energy conversion (2012)
  • Author(s): M.Iwaki
  • Organizer: Workshop : Active Dynamics on Microscales
  • Place of Presentation: Lorentz Center, Netherland
  • Year and Date: 20120916-19
  • Invited
• [Presentation] ミオシンXI の1分子解析 (2012)
  • Author(s): 岩城光宏, 他
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学、兵庫
  • Year and Date: 20120915-17
  • Invited
• [Presentation] ATP 駆動蛋白質の化学力学変換機構の1分子解析 (2012)
  • Author(s): 岩城 光宏
  • Organizer: 新学術領域合同公開シンポジウム
  • Place of Presentation: 大阪ガーデンパレス、大阪
  • Year and Date: 20120914-15
• [Presentation] Brownian search-and-catch mechanism for myosin-VI steps (2011)
  • Author(s): M.Iwaki
  • Organizer: Gordon research conferences -Stochastic physics in biology-
  • Place of Presentation: Ventura, California
  • Year and Date: 20110123-29
• [Presentation] Observation of mechanical process for myosin-II by a dark-field microscopy combined with an optical trap (2011)
  • Author(s): M.Iwaki
  • Organizer: Biophysical Society 55^<th> Annual meeting
  • Place of Presentation: Baltimore, Maryland
  • Year and Date: 2011-03-05
• [Presentation] Effect of Cosolvent on Brownian search-and-catch mechanism for actomyosin (2010)
  • Author(s): M.Iwaki
  • Organizer: International Symposium on Hydration and ATP energy
  • Place of Presentation: 仙台
  • Year and Date: 20100308-10
• [Presentation] Brownian search-and-catch mechanism for myosin-VI steps (2010)
  • Author(s): M.Iwaki
  • Organizer: Biophysical society 54^<th> annual meeting
  • Place of Presentation: San Francisco
  • Year and Date: 20100220-24
• [Presentation] 共溶媒の効果:ATP 駆動蛋白質の1分子計測の場合 (2010)
  • Author(s): 岩城光宏
  • Organizer: 日本物理学会第65回年次大会
  • Place of Presentation: 岡山大学、岡山
  • Year and Date: 2010-03-22
  • Invited
• [Presentation] ナノ粒子を利用したモーター蛋白質の高速1 分子イメージングと操作 (2009)
  • Author(s): 岩城光宏
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場、兵庫
  • Year and Date: 2009-10-21
  • Invited
• [Book] 筋機能改善の理学療法とそのメカニズム (2014)
  • Author(s): 岩城 光宏
  • Publisher: ナップ (印刷中)
• [Book] Engineering of Chemical Complexity II (2014)
  • Author(s): M.Iwaki
  • Publisher: World Scientific (in press)
• [Book] Engineering of Chemical Complexity (2013)
  • Author(s): M.Iwaki, L.Marcucci, Y.Togashi, T.Yanagida
  • Total Pages: 79-100
  • Publisher: World Scientific
• [Remarks] ① 岩城光宏、日本経済新聞「筋肉を収縮させる力 分子センサーが制御」2009年5月 報道関連情報
• [Remarks] ② Mitsuhiro Iwaki, 大阪大学出版会、「Annual Report of Osaka University -Academic Achievement- 2009-2010」、2010年、1頁 報道関連情報
• [Remarks]
  • URL: http://www.qbic.riken.jp/cdo/iwaki-subg/
• [Patent(Industrial Property Rights)] ポリヌクレオチドを用いたコイル及びその製造方法 (2013)
  • Inventor(s): 岩城 光宏
  • Industrial Property Rights Holder: 岩城 光宏
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願2013-042322
  • Filing Date: 2013-03-04