Optimization and sophistication of stimulated Raman microscope for functional Raman probes

2022 Fiscal Year Final Research Report

• Project Area: Innovative multiplex imaging with functional Raman probes
• Project/Area Number: 20H05725
• Research Category: Grant-in-Aid for Transformative Research Areas (B)
• Allocation Type: Single-year Grants
• Review Section: Transformative Research Areas, Section (II)
• Research Institution: The University of Tokyo
• Principal Investigator: Ozeki Yasuyuki 東京大学, 大学院工学系研究科(工学部), 教授 (60437374)
• Project Period (FY): 2020-10-02 – 2023-03-31
• Keywords: ラマンプローブ / 誘導ラマン散乱 / activatableプローブ / 超解像イメージング

Outline of Final Research Achievements

In this study, we have optimized and advanced the high-speed stimulated Raman scattering imaging system that we developed in order to achieve multiplex enzyme imaging, multiplex in vivo imaging, and multiplex-super-resolution imaging using the functional Raman probes created in this research field. Then we demonstrated the usefulness of these imaging methods through applications in biology. Specifically, we successfully demonstrated the use of activatable Raman probes created by the Kamiya team, demonstrated the photoswitchable Raman probes with commercially available photochromic molecules, demonstrated super-resolution imaging with high-performance photoswitchable Raman probes developed by Kamiya team, and demonstrated amino acid metabolic imaging with deuterium labeling in collaboration with Obata team.

Free Research Field

生体イメージング

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、分子の電子・分子振動の光応答を周囲環境や光照射によって制御するという新しい原理を用いた生体観察手法を創出するものです。これにより、生体内の多数の構成要素を同時に観察することが可能となり、複雑な生体の仕組みを解明する新しい手法を実現できました。本研究成果は、生物学や医学の様々な分野において生命の仕組みや病気の解明の研究に活用されることが期待できます。