2022 Fiscal Year Final Research Report
Understanding parametric translation regulation underlying neuronal functions
| Project Area | Parametric biology based on translation rate regulatory mechanism |
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| Project/Area Number |
20H05786
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| Research Category |
Grant-in-Aid for Transformative Research Areas (B)
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| Allocation Type | Single-year Grants |
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| Review Section |
Transformative Research Areas, Section (III)
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2020-10-02 – 2023-03-31
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| Keywords | オルガノイド / リボソーム / タンパク質合成 / 神経 |
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| Outline of Final Research Achievements |
To understand how the circuit structure of the cerebral connectoid, which connects two cerebral organoids via an axonal bundle, changes neuronal activity, we investigated changes in the internal cellular state focusing on protein synthesis. As a result, we found that the amount of membrane proteins that control axons is increased in connectoids compared to assemblebroids which are two cerebral organoids directly attached to each other. Given that this protein could be the key to controlling the state of the internal network of connectoids, they regulated its activity with agonists and antagonists, and found that it could be used as a method to control the exchange of neural activity between organoids.
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| Free Research Field |
神経科学
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| Academic Significance and Societal Importance of the Research Achievements |
神経回路構造を変化させることで神経活動が変化するが、それに伴って遺伝子発現も変化することを示した。この遺伝子発現が神経活動の制御を行なっており、神経回路の構造、活動、遺伝子発現のそれぞれが密接に絡み合っている様子を試験管内で観察することができた。この知見を実際の脳内の回路に当てはめて検討することによって脳の機能の基盤的機構を調べることができるだろう。また、オルガノイド間の神経活動のやりとりを調節することができたため、同様の手法で脳内の活動を変化させ、領域間の機能的な関連や協調について調べるために役立つと考えられる。
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