Synthesis of Functional Molecular Systems using Protein Nanotubes

2013 Fiscal Year Final Research Report

• Project Area: Coordination Programming - Science of Molecular Superstructures for Chemical Devices
• Project/Area Number: 21108013
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Chuo University (2010-2013); Waseda University (2009)
• Principal Investigator: KOMATSU Teruyuki 中央大学, 理工学部, 教授 (30298187)
• Research Collaborator: AKIYAMA Motofusa 中央大学, 理工学部, 助教 (90467697) ; CURRY Stephen Imperial College London, 教授 ; BOTTCHER Christoph Freie Universität Berlin, 博士
• Project Period (FY): 2009-07-23 – 2014-03-31
• Keywords: 高分子錯体 / 蛋白質 / 金属蛋白質 / ナノチューブ / クラスター / 交互積層 / 分子捕捉 / 人工酸素運搬体

Research Abstract

We synthesized cylindrical hollow structured nanotubes and core-shell structured nanoclusters by function hierarchical organization of plural proteins. New functionalities based on molecular arrangements appeared, and functional molecular systems have been constructed. The results on (i) artificial metalloproteins, (ii) protein nanotubes, and (iii) protein clusters were published as 22 research papers. Topics were highlighted by newspapers and Chemistry World (Royal Society of Chemistry), and are attracted considerable attention.

Research Products

• [Journal Article] Fluorescent Dimer and Fiber of meso-Tetrakis{o-(isonicotinoylamino)phenyl}porph yrin Connected by Pd(II) Coordinations (2014)
  • Author(s): S.Kasuya, Y.Yamazawa, N.Sugimura, T.Shibue, T.Komatsu
  • Journal Title: Chem. Lett Volume: 43 (掲載決定)
  • DOI: 10.1246/cl.140243
  • Peer Reviewed
• [Journal Article] Superoxide Dismutase Activity of the Naturally Occurring Human Serum Albumin-Copper Complex without Hydroxyl Radical Formation (2014)
  • Author(s): R.Kato, M.Akiyama, H.Kawakami, T.Komatsu
  • Journal Title: Chem. Asian J. Volume: 9 Pages: 83-86
  • DOI: 10.1002/asia.201301285
  • Peer Reviewed
• [Journal Article] Gold Nanoparticle Inclusion into Protein Nanotube as a Layered Wall Component (2013)
  • Author(s): S.Goto, Y.Amano, M.Akiyama, C.Bottcher, T.Komatsu
  • Journal Title: Langmuir Volume: 29 Pages: 14923-14930
  • DOI: 10.1021/la403283x
  • Peer Reviewed
• [Journal Article] Human Serum Albumin Mutants Complexed Mn(III) Protoporphyrin IX as Superoxide Dismutase Mimics (2013)
  • Author(s): R.Kato, Y.Kobayashi, M.Akiyama, T.Komatsu
  • Journal Title: Dalton Trans Volume: 42 Pages: 15889-15892
  • DOI: 10.1039/C3DT51418H
  • Peer Reviewed
• [Journal Article] Covalent Core-Shell Architecture of Hemoglobin and Human Serum Albumin as an Artificial O_2 Carrier (2013)
  • Author(s): D.Tomita, T.Kimura, H.Hosaka, Y.Daijima, R.Haruki, Kai Rudwig, C.Bottcher, T.Komatsu
  • Journal Title: Biomacromolecules Volume: 14 Pages: 1816-1825
  • DOI: 10.1021/bm400204y
  • Peer Reviewed
• [Journal Article] Human Serum Albumin-Based Peroxidase Having an Iron-Protoporphyrin IX in Artificial Hemepocket (2012)
  • Author(s): K.Watanabe, N.Ishikawa, T.Komatsu
  • Journal Title: Chem. Asian J. Volume: 7 Pages: 2534-2537
  • DOI: 10.1002/asia.201200373
  • Peer Reviewed
• [Journal Article] Protein-Based Nanotubes for Biomedical Applications (2012)
  • Author(s): T.Komatsu
  • Journal Title: Nanoscale Volume: 4(Feature Article, Inside Front Cover) Pages: 1910-1918
  • DOI: 10.1039/C1NR11224D
  • Peer Reviewed
• [Journal Article] Human Serum Albumin Nanotubes with Esterase Activity (2012)
  • Author(s): T.Komatsu, T.Sato, C.Bottcher
  • Journal Title: Chem. Asian J. Volume: 7 Pages: 201-206
  • DOI: 10.1002/asia.201100606
  • Peer Reviewed
• [Journal Article] Protein Nanotubes Arrays Immobilized on Solid Substrates : Molecular Trap in Aqueous Medium (2011)
  • Author(s): R.Kato, T.Komatsu
  • Journal Title: Chem. Lett. Volume: 40 Pages: 1338-1339
  • DOI: 10.1246/cl.2011.1338
  • Peer Reviewed
• [Journal Article] Virus Trap in Human Serum Albumin Nanotube (2011)
  • Author(s): T.Komatsu, X.Qu, H.Ihara, M.Fujihara, H.Azuma, H.Ikeda
  • Journal Title: J. Am. Chem. Soc. Volume: 133 Pages: 3246-3248
  • DOI: 10.1021/ja1096122
  • Peer Reviewed
• [Journal Article] Protein Nanotubes with an Enzyme Interior Surface (2011)
  • Author(s): T.Komatsu, H.Terada, N.Kobayashi
  • Journal Title: Chem. Eur. J. Volume: 17 Pages: 1849-1854
  • DOI: 10.1002/chem.201001937
  • Peer Reviewed
• [Journal Article] Solid Nanotubes Comprising α-Fe_2O_3 Nanoparticles Prepared from Ferritin Protein (2010)
  • Author(s): X.Qu, N.Kobayashi, T.Komatsu
  • Journal Title: ACS Nano Volume: 4 Pages: 1732-1738
  • DOI: 10.1021/nn901879d
  • Peer Reviewed
• [Journal Article] Molecular Capture in Protein Nanotubes (2010)
  • Author(s): X.Qu, T.Komatsu
  • Journal Title: ACS Nano Volume: 4 Pages: 563-573
  • DOI: 10.1021/nn901474y
  • Peer Reviewed
• [Journal Article] The Role an Amino Acid Triad at the Entrance of the Heme Pocket in Human Serum Albumin for O_2 and CO Binding to Iron Protoporphyrin IX (2009)
  • Author(s): T.Komatsu, A.Nakagawa, S.Curry, E.Tsuchida, K.Murata, N.Nakamura, H.Ohno
  • Journal Title: Org. Biomol. Chem. Volume: 7 Pages: 3836-3841
  • DOI: 10.1039/B909794E
  • Peer Reviewed
• [Journal Article] Structural and Mutagenic Approach to Create Human Serum Albumin-Based Oxygen Carrier and Photosensitizer (2009)
  • Author(s): T.Komatsu, A.Nakagawa, X.Qu
  • Journal Title: Drug Metab. Pharacokinet Volume: 24 Pages: 287-299
  • DOI: 10.2133/dmpk.24.287
  • Peer Reviewed
• [Journal Article] Artificial Oxygen Carriers, Hemoglobin Vesicles and Albumin-Hemes Based on Bioconjugate Chemistry (2009)
  • Author(s): E.Tsuchida, K.Sou, A.Nakagawa, H.Sakai, T.Komatsu, K.Kobayashi
  • Journal Title: Bioconjugate Chem. Volume: 20(Review, Cover Image) Pages: 1419-1440.
  • DOI: 10.1021/bc800431d
  • Peer Reviewed
• [Presentation] Core-Shell Architecture of Hemoglobin and Human Serum Albumin as an Artificial O_2 Carrier (2014)
  • Author(s): T.Komatsu
  • Organizer: International Symposium on Coordination Programming 2014
  • Place of Presentation: Tokyo
  • Year and Date: 2014-01-21
  • Invited
• [Presentation] Protein Nanotubes for Biomedical Applications (2013)
  • Author(s): T.Komatsu
  • Organizer: 12^<th> International Conference on Polymers for Advanced Technologies
  • Place of Presentation: Berlin (Germany)
  • Year and Date: 2013-10-02
  • Invited
• [Presentation] Protein Nanotubes for Biomedical and Catalytic Applications (2013)
  • Author(s): T.Komatsu
  • Organizer: IUPAC 15^<th> International Symposium on MacroMolecular Complexes
  • Place of Presentation: Greenville, SC (USA)
  • Year and Date: 2013-08-15
  • Invited
• [Presentation] Artificial Hemoproteins Comprising Human Serum Albumin Mutants Complexed with Iron Protoporphyrin IX (2012)
  • Author(s): T.Komatsu
  • Organizer: 7^<th> International Conference on Porphyrins and Phthalocyanines
  • Place of Presentation: Jeju (Korea)
  • Year and Date: 2012-07-07
  • Invited
• [Presentation] Artificial Hemoproteins Comprising Human Serum Albumin Complexed with Iron Protoporphyrin IX in a Tailor-Made Heme Pocket (2012)
  • Author(s): T.Komatsu
  • Organizer: 12^<th> Eurasia Conference on Chemical Sciences
  • Place of Presentation: Corfu (Greece)
  • Year and Date: 2012-04-21
  • Invited
• [Presentation] Biomolecular Capture in Protein Nanotubes (2011)
  • Author(s): T.Komatsu
  • Organizer: 2011 East Asian Symposium on Polymers for Advanced Technology
  • Place of Presentation: Jeju (Korea)
  • Year and Date: 2011-06-14
  • Invited
• [Presentation] Protein Nanotubes for Biomolecular Separation (2009)
  • Author(s): T.Komatsu, X.Qu
  • Organizer: 5^<th> IUPAC International Conference on Novel Materials and their Synthesis 19^<th> International Symposium on Fine Chemistry and Functional Polymers
  • Place of Presentation: Shanghai (China)
  • Year and Date: 2009-10-21
  • Invited
• [Book] Albumin-Heme Oxygen Carriers Hemoglobin-Based Oxygen Carriers as Red Cell Substitutes and Oxygen Therapeutics, Eds. H. W. Kim, A. G. Greenburg (Chapter 18) (2013)
  • Author(s): T.Komatsu
  • Total Pages: 339-348
  • Publisher: Springer-Verlag
• [Remarks] 研究代表者が中央大学へ異動(2010年度)したのを契機に、中央大学理工学部応用化学科のweb サイト内に「小松研究室」ホームページ(日本語版と英語版)を開設、本研究で得られた成果を随時国内外に向け発信している。 (1)ホームページ
  • URL: http://www.chem.chuo-u.ac.jp/~komatsu-lab/index.html
• [Remarks] ① 日経産業新聞2013年7月24日"たんぱく質からナノチューブ-中大が作成技術-" (2)研究紹介・記事
• [Remarks] ② 日刊工業新聞2013年5月14日 "人工酸素運搬体新たに合成" (2)研究紹介・記事
• [Remarks] ③ 日経バイオテク2013年5月14日 "中央大の小松晃之教授ら、ヘモグロビンをアルブミンで包み込んだ人工酸素運搬体の成果を論文発表" (2)研究紹介・記事
• [Remarks] ④ マイナビニュース2013年5月14日 "中央大、製造しやすくて常温で長持ちして血液型を選ばない人工血液を開発" (2)研究紹介・記事
• [Remarks] ⑤ プレスリリース(中央大学)2013年5月13日 "酸素を輸送できるタンパク質クラスターの合成に成功" (2)研究紹介・記事
• [Remarks] ⑥ Chemistry World (Royal Society of Chemistry), 2011, February. "Protein nanotubes traps viruses" (2)研究紹介・記事
• [Patent(Industrial Property Rights)] ヘモグロビン-アルブミン複合体、並びに該複合体を含む人工血漿増量剤及び人工酸素運搬体 (2012)
  • Inventor(s): 小松晃之、冨田大樹
  • Industrial Property Rights Holder: 中央大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: PCT/JP2012/001118
  • Filing Date: 2012-02-20
  • Overseas