2013 Fiscal Year Final Research Report
Elucidating the allorecognition mechanisms in ascidians (prochordates)
| Project Area | Elucidation of Common Mechanisms for Allogeneic Authentication in Animals and Plants |
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| Project/Area Number |
21112002
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagoya University |
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Principal Investigator |
SAWADA Hitoshi 名古屋大学, 理学(系)研究科(研究院), 教授 (60158946)
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| Project Period (FY) |
2009-07-23 – 2014-03-31
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| Keywords | ホヤ / 受精 / 精子 / 卵 / 自家不和合性 / 自己非自己認識 |
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| Research Abstract |
Ascidians (Protochordates) are hermaphrodities, which release sperm and eggs almost simultaneously. Several species, including Ciona intestinalis, show self-incompatibility to avoid self-fertilization. We revealed that three pairs of egg-side ligands on the vitelline-coat (v-Themis-A, -B, and -B2) and cognate sperm-side receptors (s-Themis-A, -B, and -B2) are responsible for self/non-self recognition (allorecognition) during fertilization in C. intestinalis by genetic analyses, proteomic analyses, and genome-editing techniques. We also found that when C. intestinalis spermatozoon binds to the vitelline coat of a self-egg, Ca2+ influx into sperm takes place, resulting in the detachment of sperm from the vitelline coat. Since s-Themis-B/B2 contain a cation channel domain in their C-terminal regions, s-Themis-B/B2 may play a key role in self-recognition during fertilization of the ascidian C. intestinalis.
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[Journal Article] Integrative omics analysis reveals differentially distributed proteins in dimorphic euspermatozoa of the squid, Loligo bleekeri2014
Author(s)
Yoshida, M.A., Yamada, L., Ochi, H., Iwata, H., Tamura-Nakano, M., Sawada H., Sauer, W.H., Ogura, A., and Hirohashi, M.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: (in press)
DOI
Peer Reviewed
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[Journal Article] Glutathione transferase theta in apical ciliary tuft regulates mechanical reception and swimming behavior of sea urchin embryos2013
Author(s)
Jin, Y., Yaguchi, S., Shiba, K., Yamada, L., Yaguchi, J., Shibata, D. Sawada, H., and Inaba, K.
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Journal Title
Cytoskeleton
Volume: 70(8)
Pages: 453-470
DOI
Peer Reviewed
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[Journal Article] A surface glycoprotein indispensable for gamete fusion in the social amoeba Dictyostelium discoideum2012
Author(s)
Araki, Y., Shimizu, H.D., Saeki, K., Okamoto, M., Yamada, L., Ishida, K., Sawada, H., and Urushihara, H.
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Journal Title
Eukaryot. Cell
Volume: 11
Pages: 638-644
DOI
Peer Reviewed
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[Journal Article] Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nuclease2012
Author(s)
Kawai, N., Ochiai, H., Sakuma, T., Yamada, L., Sawada, H., Yamamoto, T., and Sasakura, Y.
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Journal Title
Dev. Growth Differ
Volume: 54
Pages: 536-545
DOI
Peer Reviewed
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[Journal Article] Identification and localization of sperm CRISP family protein CiUrabin involved in gamete interaction in the ascidian Ciona intestinalis2011
Author(s)
Yamaguchi, A., Saito, T., Yamada, L., Taniguchi, H., Harada, Y., and Sawada, H.
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Journal Title
Mol. Reprod. Dev.
Volume: 78
Pages: 488-497
DOI
Peer Reviewed
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[Book] Allorecognition and lysisn systems during ascidian fertilization. In Sexual Reproduction in Animals and Plants, (H.Sawada, N.Inoue, M.Iwano (eds.))2014
Author(s)
Sawada, H, Yamamoto, K., Otsuka, K., Saito, T., Yamaguchi, A., Mino, M., Akasaka, M., Harada, Y., and Yamada, L.
Total Pages
231-244
Publisher
Springer
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[Book] Novel isoform of vitellogenin expressed in eggs is a binding partner of the sperm proteases, HrProacrosin and HrSpermosin, in the ascidian, Halocynthia roretzi. In Sexual Reproduction in Animals and Plants, (H.Sawada, N.Inoue, M.Iwano (eds.))2014
Author(s)
Akasaka, M., Kato, K. H., Kitajima, K., Sawada, H.
Total Pages
131-139
Publisher
Springer
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[Book] Allorecogntion mechanisms in ascidian fertilization : A reproduction strategy shared with flowering plants. In Sperm Cell Research in the 21st Century : Historical Discoveries to New Horizons, (M.Morisawa (ed.))2012
Author(s)
Sawada, H., Saito, T., Yamaguchi, A., Harada, Y., and Yamada, L.
Total Pages
202-207
Publisher
Adthree Publishing Co
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