Authentication mechanism of mammalian sperm in the female reproductive tract

2013 Fiscal Year Final Research Report

• Project Area: Elucidation of Common Mechanisms for Allogeneic Authentication in Animals and Plants
• Project/Area Number: 21112005
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Tsukuba
• Principal Investigator: BABA TADASHI 筑波大学, 生命環境系, 教授 (40165056)
• Project Period (FY): 2009-07-23 – 2014-03-31
• Keywords: マウス / 精子 / 卵子 / 卵管 / 卵丘細胞塊

Research Abstract

This study describes the authentication mechanism of fertilizing sperm during migration through the female reproductive tract. We have successfully identified a sperm ADAM3-interacting membrane protein localized on epithelial cells in the uterus and oviduct. Functional analysis of the membrane protein suggests a possible involvement in release of sperm from the epithelial cells. We also found that double-knockout mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, although the mutant epididymal sperm fail to undergo the acrosome reaction on the zona surface and to traverse the zona pellucida in vitro. Moreover, We have identified a novel proteasome subunit specifically present in sperm. The function of the mouse sperm proteasome may be distinguished from sea urchin and starfish proteasomes by functional diversification.

Research Products

• [Journal Article] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in the mouse (2013)
  • Author(s): Kanemori, Y., Ryu, J.-H., Sudo, M., Niida-Araida, Y., Kodaira, K., Takenaka, M., Kohno, N., Sugiura, S., Kashiwabara, S., and Baba, T.
  • Journal Title: Biol. Reprod Volume: 88 Pages: 105, 1-8
  • DOI: 10.1095/biolreprod.112.107425
  • Peer Reviewed
• [Journal Article] Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization (2012)
  • Author(s): Zhou, C., Kang, W., and Baba, T.
  • Journal Title: J. Reprod. Dev Volume: 58 Pages: 330-337
  • URL: https://www.jstage.jst.go.jp/article/jrd/58/3/58_2011-006/_article
  • Peer Reviewed
• [Journal Article] Phosphatidylinositol 4-phosphate 5-kinase is indispensable for mouse spermatogenesis (2012)
  • Author(s): Hasegawa, H., Noguchi, J., Yamashita, M., Okada, R., Sugimoto, R., Furuya, M., Unoki, T., Funakoshi, Y., Baba, T., and Kanaho, Y.
  • Journal Title: Biol. Reprod Volume: 86 Pages: 1-12
  • DOI: 10.1095/biolreprod.110.089896
  • Peer Reviewed
• [Journal Article] Possible involvement of mitogen- and stress-activated protein kinase 1, MSK1, in metaphase-ll arrest through phosphorylation of EMI2 in mouse oocytes (2011)
  • Author(s): Miyagaki, Y., Kanemori, Y., and Baba, T.
  • Journal Title: Dev. Biol Volume: 359 Pages: 73-81
  • DOI: 10.1016/j.ydbio.2011.08.021
  • Peer Reviewed
• [Journal Article] Expression analysis of MIF4GD in the rat testis (2011)
  • Author(s): Okada, K., Kimura, M., Moriyama, Y., Nakai, M., Kikuchi, K., Kaneko, H., Kunieda, T., Baba, T., and Noguchi, J.
  • Journal Title: J. Reprod. Dev Volume: 57 Pages: 256-261
  • URL: https://www.jstage.jst.go.jp/article/jrd/57/2/57_10-138H/_article
  • Peer Reviewed
• [Journal Article] Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme (2010)
  • Author(s): Akama, K., Horikoshi, T., Sugiyama, A., Nakahata, S., Akitsu, A., Niwa, N., Intoh, A., Kakui, Y., Sugaya, M., Takei, K., Imaizumi, N., Sato, T., Matsumoto, R., Iwahashi, H., Kashiwabara, S., Baba, T., Nakamura, M., and Toda, T.
  • Journal Title: Biochim. Biphys. Acta Volume: 1804 Pages: 1272-1284
  • DOI: 10.1016/j.bbapap.2010.02.004
  • Peer Reviewed
• [Journal Article] Mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, but the mutant sperm are infertile in vitro (2010)
  • Author(s): Kawano, N., Kang, Wj., Yamashita, M., Koga, Y., Yamazaki, T., Hata, T., Miyado, K., and Baba, T.
  • Journal Title: Biol. Reprod Volume: 83 Pages: 359-369
  • DOI: 10.1095/biolreprod.109.083089
  • Peer Reviewed
• [Journal Article] Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization? (2009)
  • Author(s): Kang,WJ., Zhou, C., Koga, Y., and Baba, T.
  • Journal Title: J. Reprod. Dev Volume: 56 Pages: 140-144
  • URL: https://www.jstage.jst.go.jp/article/jrd/56/1/56_09-152N/_article
  • Peer Reviewed
• [Journal Article] Functional roles of sperm hyaluronidase, HYAL5 and SPAM1, in mouse fertilization (2009)
  • Author(s): Kimura, M., Kim, E., Kang,WJ., Yamashita, M., Saigo, M., Yamazaki, T., Nakanishi, T., Kashiwabara, S., and Baba, T.
  • Journal Title: Biol. Reprod Volume: 81 Pages: 939-947
  • DOI: 10.1095/biolreprod.109.078816
  • Peer Reviewed
• [Presentation] 精子アクロソームタンパク質ACRBP とACRのダブル欠損マウスの解析 (2013)
  • Author(s): 高嶋明日登,兼森芳紀,首藤舞,康宇鎮,馬場忠
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2013-12-04
• [Presentation] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in mouse spermatogenesis (2013)
  • Author(s): Sudo, M., Kanemori, Y., Ryu, JH., Niida-Araida, Y., Kodaira, K., Takenaka, M., Kohno, N., Sugiura, S., Kashiwabara, S., and Baba, T.
  • Organizer: Gordon Research Conference Program on Fertilization & Activation of Development
  • Place of Presentation: Holderness School Holderness, NH, USA
  • Year and Date: 2013-07-15
• [Presentation] Proacrosin-binding protein ACRBP/sp32 regulates acrosome formation during mouse spermiogenesis (2013)
  • Author(s): Kanemori, Y., Sudo, M., Koga, Y., Okabe, M., and Baba, T.
  • Organizer: Gordon Research Conference Program on Fertilization &Activation of Development
  • Place of Presentation: Holderness School Holderness, NH, USA
  • Year and Date: 2013-07-15
• [Presentation] Functional role of mouse ACRBP/sp32 in spermatogenesis and fertilization (2012)
  • Author(s): 柳 辰協、首藤 舞、兼森 芳紀、柏原 真一、馬場 忠
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-14
• [Presentation] Sperm penetration through the cumulus matrix (2012)
  • Author(s): Baba, T
  • Organizer: International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: 名古屋ガーデンパレス
  • Year and Date: 2012-11-13
• [Presentation] Localization of a PSMA8-containing proteasome in mouse sperm (2011)
  • Author(s): Koyama, M., Koga, Y., Hyodo, H., Yamashita, M., Ogichi, K., Kanemori, Y., Sawada H., and Baba, T.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] 精子の卵丘細胞層通過でのヒアルロニダーゼとセリンプロテアーゼによる協働的な作用 (2011)
  • Author(s): 周崇、康宇鎭、馬場忠
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] Characterization of a novel PSMA8-containing proteasome present in mouse sperm (2011)
  • Author(s): Koga, Y., Koyama, M., Hyodo, H., Yamashita, M., Sawada, H., Baba, T.
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: ケアンズコンベンションセンター
  • Year and Date: 2011-10-10
• [Presentation] Acrosome reaction of mouse epididymal sperm facilitates the entry into the cumulus mass (2011)
  • Author(s): Kang,Wj., Zhou, C., Yamashita, M., and Baba, T.
  • Organizer: 動植物アロ認証 第3回領域会議
  • Place of Presentation: 関西セミナーハウス、京都市
  • Year and Date: 2011-06-30
• [Presentation] Fuctional Analysis of PABPC2 in Mouse Testis (2011)
  • Author(s): Miyagaki, Y., Kimura, M., Ishida, K., Kashiwabara SI., and Baba, T.
  • Organizer: 2011 XXIst North American TestisWorkshop
  • Place of Presentation: Montrral QC, Canada
  • Year and Date: 2011-03-31
• [Presentation] 受精とプロテオリシス (2010)
  • Author(s): 馬場 忠
  • Organizer: 第33回日本分子生物学会年会ワークショップ「動植物に共通するアロ認証中核原理を探る」
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2010-12-07
• [Presentation] The trypsin-like serine protease activity of ACR and PRSS21 is important but not essential for fertilization in vivo (2010)
  • Author(s): Kang, Wj., Kawano, N., Yamashita, M., Koga, Y., Yamazaki, T., Hata, T., Miyado, k., and Baba, T.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] The trypsin-like serine protease activity of ACR and PRSS21 is important but not essential for fertilization in vivo (2010)
  • Author(s): Kang,Wj., Kawano, N., Yamashita, M., Koga, Y., Yamazaki, T., Hata, T., Miyado, K., and Baba, T.
  • Organizer: Illinois Symposium on Reproductive Sciences in Health and Disease
  • Place of Presentation: University of Illinois, Chicago, USA
  • Year and Date: 2010-10-11
• [Presentation] Possible role of mitogen- and stress-activated protein kinase-1 (MSK1) in meiotic arrest of mouse oocytes (2010)
  • Author(s): Miyagaki, Y., Kanemori, Y., and Baba, T.
  • Organizer: Gordon Research Conference
  • Place of Presentation: New Hampshire, USA
  • Year and Date: 2010-08-16
• [Presentation] Effects of placental scars on implantation in multiparous mice (2010)
  • Author(s): Yamashita, M., and Baba, T.
  • Organizer: Gordon Research Conference Program on Reproductive Tracts Biology
  • Place of Presentation: New Hampshire, USA
  • Year and Date: 2010-08-16
• [Presentation] Functional analysis of sperm proteasome in mouse fertilization (2009)
  • Author(s): Hyodo, H., Yamashita, M., Izumi, Y., Sawada, H., and Baba, T.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization? (2009)
  • Author(s): Kang,WJ., Zhou, C., Koga, Y., and Baba, T.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] Recognition system of mammalian sperm in the female reproductive tract (2009)
  • Author(s): Baba, T.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] Target proteins of SBTI in sperm acrosome reaction (2009)
  • Author(s): Izumi, Y., Yamashita, M., Hyodo, H., Kang, WJ., and Baba, T.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] Role of hyaluronidase in sperm penetration through cumulus matrix (2009)
  • Author(s): Baba, T.
  • Organizer: 20^<th> International Symposium on Glycoconjugates
  • Place of Presentation: San Juan, Puerto Rico (USA)
  • Year and Date: 2009-11-30
• [Remarks] 馬場 忠研究室
  • URL: http://www.acroman.org/