2013 Fiscal Year Final Research Report
Structural analyses of the metastable ligand-receptor interactions
| Project Area | Integrative understanding of biological processes mediated by transient macromolecular complexes; New technology for visualizing physiologically metastable states. |
|---|
| Project/Area Number |
21121002
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Complex systems
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
SHIMADA Ichio 東京大学, 薬学研究科(研究院), 教授 (70196476)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
JEE Jungoo 首都大学東京, 戦略研究センター, 准教授 (50381554)
KAINOSHO Masatsune 首都大学東京, 戦略研究センター, 客員教授 (20137029)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
OSAWA Masanori 東京大学, 大学院薬学系研究科, 講師 (60361606)
UEDA Takumi 東京大学, 大学院薬学系研究科, 助教 (20451859)
NISHIDA Noritaka 東京大学, 大学院薬学系研究科, 助教 (50456183)
KAINOSHO Masatsune 首都大学東京, 戦略研究センター, 教授 (20137029)
|
|---|
| Project Period (FY) |
2009-07-23 – 2014-03-31
|
| Keywords | 核磁気共鳴法 / 膜タンパク質 / 相互作用 / 過渡的複合体 |
|---|
| Research Abstract |
It has been considered that the transient molecular complexes play an important role in many biological processes inside the living cells. In this project, we aimed to establish the NMR methodologies, which can observe the NMR signals from such short lived states, and reconstitute such state by mimicking physiological environments. We applied these methods to the actual biologically important systems, including chemokine-chemokine receptors, the cell adhesion molecule CD44, and the photosynthesis complex, and successfully obtained the structural information of the transiently formed complex of those molecules, which are related to their function.
|
-
-
-
-
-
-
-
-
[Journal Article] Two-state conformations in the hyaluronan-binding domain regulate CD44 adhesiveness under flow condition2010
Author(s)
Ogino S, Nishida N, Umemoto R, Suzuki M, Takeda M, Terasawa H, Kitayama J, Matsumoto M, Hayasaka H, Miyasaka M, Shimada I
-
Journal Title
Structure.
Volume: 18(5)
Pages: 649-56
DOI
-
-
[Journal Article] Structural Basis of the Interaction between Chemokine Stromal Cell-derived Factor-1/CXCL12 and Its G-protein-coupled Receptor CXCR42009
Author(s)
Kofuku Y, Yoshiura C, Ueda T, Terasawa H, Hirai T, Tominaga S, Hirose M, Maeda Y, Takahashi H, Terashima Y, Matsushima K, Shimada I
-
Journal Title
J Biol Chem.
Volume: 284(50)
Pages: 35240-50
DOI
-
-
-
-
-
-
-
-
-
-
-
-
-
-
