Chemical glycan modification of membrane proteins for "manipulating" membrane dynamics

2023 Fiscal Year Final Research Report

• Project Area: Regulation of membrane dynamics by glycan chemical knock-in
• Project/Area Number: 21H05077
• Research Category: Grant-in-Aid for Transformative Research Areas (B)
• Allocation Type: Single-year Grants
• Review Section: Transformative Research Areas, Section (II)
• Research Institution: National Institute of Advanced Industrial Science and Technology (2022-2023); The University of Tokyo (2021)
• Principal Investigator: Kounosuke Oisaki 国立研究開発法人産業技術総合研究所, 材料・化学領域, 研究チーム長 (00583999)
• Co-Investigator(Kenkyū-buntansha): 川島 茂裕 東京大学, 大学院薬学系研究科(薬学部), 准教授 (40508115) ; 金井 求 東京大学, 大学院薬学系研究科(薬学部), 教授 (20243264)
• Project Period (FY): 2021-08-23 – 2024-03-31
• Keywords: 生体適合化学 / 膜タンパク質 / 糖鎖 / 生体共役反応 / 膜動態 / エクソソーム

Outline of Final Research Achievements

Glycans add to proteins and lipids to exert their functions, and glycosylation, particularly onto membrane proteins, plays an important role in biological phenomena such as endocytosis and cell-to-cell transfer. However, the detailed mechanisms have remained unclear. This study aimed to create precise glycan-installation onto membrane proteins through the development of novel bioconjugation techniques, and to develop artificial methodologies to manipulate the membrane dynamics. As a result of the research, amino acid residue-selective modification reactions and antibody modification reactions using original reaction chemistry were developed, showing the possibility of glycan installations.

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、糖タンパク質膜ダイナミクスの精密な理解と人工的な制御を進め、生体適合性の高い化学反応（速度論的的化学摂動）を利用した、新規薬物送達系の開発や、革新的生命操作法につながる技術基盤に成ることが期待される。