2023 Fiscal Year Final Research Report
Chrono-proteinology : Seeking for functional KaiC homologues in mammals
| Project Area | Chrono-proteinology: principle and design for protein timers |
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| Project/Area Number |
21H05132
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| Research Category |
Grant-in-Aid for Transformative Research Areas (B)
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| Allocation Type | Single-year Grants |
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| Review Section |
Transformative Research Areas, Section (III)
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| Research Institution | Fukui Prefectural University (2023)
Institute for Molecular Science (2021-2022) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
八木田 和弘 京都府立医科大学, 医学(系)研究科(研究院), 教授 (90324920)
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| Project Period (FY) |
2021-08-23 – 2024-03-31
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| Keywords | 概日時計 / KaiC / 周波数特性 |
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| Outline of Final Research Achievements |
This research project aims to develop technique to search for clock proteins in mammals that define circadian timescale such as the cyanobacterial clock protein KaiC. We have developed a technique to detect circadian rhythm in a clock reconstructed system using highly sensitive Trp fluorescence probe from KaiC and succeeded in increasing the sensitivity by a factor of 10 or more. We also found that the clock protein complexes were formed at the stage of the emergence of circadian rhythms using an in vitro system that recapitulates the developmental process of mice.
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| Free Research Field |
時間生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本成果はKaiCの変異体スクリーニング系の整備により周波数特性を創出する因子（配列機能相関）の同定につながる基盤技術である。今後、マウスの発生過程において概日リズムの出現とともに形成される時計タンパク質複合体についてKaiCの知見と照合することが、哺乳類（マウス）において周波数特性を担う因子の同定につながると期待される。
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