Reconstitution of anticancer agent-biosynthetic machinery and elucidation of its mechanism on molecular diversification

2014 Fiscal Year Final Research Report

• Project Area: Biosynthetic machinery: Deciphering and regulating the system for bioactive metabolite diversification
• Project/Area Number: 22108002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Hokkaido University
• Principal Investigator: OIKAWA Hideaki 北海道大学, 理学(系)研究科(研究院), 教授 (00185175)
• Co-Investigator(Renkei-kenkyūsha): MINAMI Atsushi 北海道大学, 大学院理学研究院, 助教 (40507191) ; OGURI Hiroki 北海道大学, 大学院理学研究院, 准教授 (80311546)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: 生合成 / 麹菌 / ペプチド合成酵素 / 全生合成 / 抗腫瘍性物質

Outline of Final Research Achievements

“Total biosynthesis” is a novel approach to provide natural products. To achieve total biosynthesis of fungal metabolites, Aspergillus oryzae expression system is one of a promising tool due to highly reliable expression of biosynthetic enzyme genes. Complete stepwise reconstitution of biosynthetic machinery is a key issue of total biosynthesis. This provides not only information of complete reaction sequence but target natural product together with all reaction intermediates which allow us to study enzymatic mechanism of a specific step. We have achieved genome mining, total biosynthesis of structurally unique metabolites and elucidation of complex reaction mechanism of modification enzymes.

Free Research Field

生物有機化学、ケミカルバイオロジー