Studies on biosynthetic assembly line of meroterpenoids

2014 Fiscal Year Final Research Report

• Project Area: Biosynthetic machinery: Deciphering and regulating the system for bioactive metabolite diversification
• Project/Area Number: 22108004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: The University of Tokyo
• Principal Investigator: ABE IKURO 東京大学, 薬学研究科(研究院), 教授 (40305496)
• Co-Investigator(Kenkyū-buntansha): MATSUDA Yudai 東京大学, 大学院薬学系研究科, 助教 (10720766)
• Co-Investigator(Renkei-kenkyūsha): WAKIMOTO Toshiyuki 東京大学, 大学院薬学系研究科, 准教授 (70363900) ; AWAKAWA Takayoshi 東京大学, 大学院薬学系研究科, 助教 (80609834) ; MORITA Hiroyuki 東京大学, 大学院薬学系研究科, 助教 (20416663)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: メロテルペノイド / 生合成 / 遺伝子クラスター / 多様性創出 / 機能制御 / 二次代謝酵素

Outline of Final Research Achievements

Meroterpenoids are hybrid natural products of both terpenoid and polyketide origin. We identified several biosynthetic gene clusters of pharmaceutically important fungal meroterpenoids, which includes that is responsible for the production of pyripyropene A, terretonin, anditomin, and andrastin, through reconstituted biosynthesis in a heterologous fungal expression system. Interestingly, the biosynthetic assembly lines are rich sources for interesting enzymes and chemistry, such as previously unknown membrane-bound terpene cyclases and multifunctional oxygenases, for the generation of structurally divergent complex fungal meroterpenoids.

Free Research Field

天然物化学、生物有機化学