Versatile host for the heterologous expression of secondary metabolism.

2014 Fiscal Year Final Research Report

• Project Area: Biosynthetic machinery: Deciphering and regulating the system for bioactive metabolite diversification
• Project/Area Number: 22108006
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Kitasato University
• Principal Investigator: IKEDA Haruo 北里大学, 感染制御科学府, 教授 (90159632)
• Co-Investigator(Kenkyū-buntansha): KOMATSU Mamoru 北里大学, 大学院感染制御科学府, 講師 (40414057)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: 二次代謝産物 / 生合成マシナリー / 異種発現 / 放線菌

Outline of Final Research Achievements

An industrial microorganism Streptomyces avermitilis, which is a producer of anthelmintic macrocyclic lactones, avermectins, has been constructed as a versatile model host for heterologous expression of genes encoding secondary metabolite biosynthesis. Twenty of the entire biosynthetic gene clusters for secondary metabolites were successively cloned and introduced into a versatile model host S. avermitilis SUKA17 or 22. Almost all S. avermitilis transformants carrying the entire gene cluster produced metabolites as a result of the expression of biosynthetic gene clusters introduced. A few transformants were unable to produce metabolites but their production was restored by the expression of biosynthetic genes using an alternative promoter or the expression of a regulatory gene in the gene cluster that controls the expression of biosynthetic genes in the cluster using an alternative promoter.

Free Research Field

微生物分子遺伝学