2015 Fiscal Year Final Research Report
Multidimendional FRET imaging of intracellular signal transduction
| Project Area | Mutli-dimensional fluorescence live imaging of cellular function and molecular activity |
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| Project/Area Number |
22113002
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KIYOKAWA Etsuko 金沢医科大学, 医学部, 教授 (80300929)
HIRATSUKA Takuya 京都大学, 医学研究科, 助教 (90641639)
OHBA Yusuke 北海道大学, 医学(系)研究科(研究院), 教授 (30333503)
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| Co-Investigator(Renkei-kenkyūsha) |
AOKI Kazuhiro 京都大学, 大学院医学研究科, 准教授 (80511427)
NAKAMURA Takeshi 東京理科大学, 大学院生命科学研究科, 教授 (60362604)
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| Project Period (FY) |
2010-04-01 – 2016-03-31
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| Keywords | がん / 二光子イメージング / フレットイメージング / 蛍光タンパク質 / バイオセンサー |
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| Outline of Final Research Achievements |
FRET biosensors visualize activities of signaling molecules in living cells. However, the use was limited mostly to the cultured 2D cells. Here, we aimed at developing technologies to apply FRET biosensors in 3D culture cells and in living tissues. Due to high recombination rates, stable expression of FRET biosensors had been a difficult task. To overcome this problem, we employed the transposon-mediated gene transfer. We found either piggcBac transposase or Tol2 trasnpoase worked efficiently for the gene transfer of FRET biosensor cDNAs. In particular, we found tol2-mediated gene transfer worked efficiently to develop transgenic mice highly-expressing FRET biosensors, which we named FRET mice. By using FRET mice, we observed activation of various signaling molecules in living tissues.
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| Free Research Field |
蛍光イメージング
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