Visualizing biological functions at many different scales

2014 Fiscal Year Final Research Report

• Project Area: Mutli-dimensional fluorescence live imaging of cellular function and molecular activity
• Project/Area Number: 22113003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MIYAWAKI Atsushi 独立行政法人理化学研究所, 脳科学総合研究センター, チームリーダー (80251445)
• Co-Investigator(Kenkyū-buntansha): HAMA Hiroshi 独立行政法人理化学研究所, 脳科学総合研究センター, 専門職研究員 (30261796) ; SASAKI Kazuki 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 ; SHIMOZONO Satoshi 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (40391982) ; NIINO Yusuke 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (10584584)
• Co-Investigator(Renkei-kenkyūsha): SAKAUE Asako 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (90462689) ; KAWANO Hiroyuki 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (10332256) ; FUKANO Takashi 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (80373364) ; ANDO Ryoko 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (10706170)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: イメージング / 蛍光たんぱく質 / 細胞周期 / 酸化ストレス / 透明化試薬 / レチノイン酸 / FRET / 細胞内結晶

Outline of Final Research Achievements

Using population and time-lapse imaging analyses of cancer cells expressing new versions of the fluorescent cell-cycle indicator, Fucci, we found great diversity in the cell-cycle alterations induced by anticancer drugs. Drug-induced cell cycle modulation varied not only between different cell types or following treatment with different drugs, but also between cells treated with different concentrations of the same drug. Using newly developed probes for retinoic acid (RA), GEPRA, we performed live imaging of zebrafish embryos at the gastrula and somitogenesis stages, which revealed a linear concentration gradient of endogenous RA in a two-tailed source-sink arrangement across the embryo. We developed a simple yet efficient method, Scale, that increases the transparency of biological tissue while preserving the signals of fluorescent labels. We established procedures for large-scale high-resolution 3D reconstruction of labeled structures within fixed specimens of millimeter size.

Free Research Field

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