Development of new intravital fluorescence imaging system and analysis of the cancer microenvironment

2014 Fiscal Year Final Research Report

• Project Area: Mutli-dimensional fluorescence live imaging of cellular function and molecular activity
• Project/Area Number: 22113004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Ehime University
• Principal Investigator: IMAMURA Takeshi 愛媛大学, 医学(系)研究科(研究院), 教授 (70264421)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: バイオテクノロジー / シグナル伝達 / 遺伝子 / 生体分子 / 細胞・組織

Outline of Final Research Achievements

We have developed a new biological fluorescence observation system in combination with a fluorescence zoom microscopy and a confocal laser microscopy. Using the mew microscopy system, cancer cells and blood vessels could be visualized in real-time from whole tumor tissues of mouse to single cell levels. I addition, we developed several cell lines that can be visualized cell cycle progression and TGF-beta signaling. Finally, cell cycle and TGF-beta signaling of cancer cells as well as protease activity of tumor microenvironment including Cathepsins and MMPs were visualized and evaluated in vivo.

Free Research Field

バイオイメージング