Two-photon imaging of cellular function in tumor immunity and immune dysregulation

2014 Fiscal Year Final Research Report

• Project Area: Mutli-dimensional fluorescence live imaging of cellular function and molecular activity
• Project/Area Number: 22113006
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: OKADA Takaharu 独立行政法人理化学研究所, その他部局等, その他 (50452272)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: イメージング / 免疫

Outline of Final Research Achievements

Germinal centers are the important site for the production of high affinity antibodies against pathogenic microbes. Our study revealed where in the lymphoid tissues B cells become committed to differentiation to germinal center B cells. Real time imaging analyses showed that the transcription factor Bcl6 plays an important role in B cell immigration into germinal centers. Imaging analyses also revealed that S1PR2, a receptor for sphingosine-1-phosophate, expressed by germinal center helper T cells is critical for their retention in germinal centers. In addition, our imaging analyses identified a subset of dendritic cells that is likely to be the most important for cytotoxic T lymphocyte development and differentiation, which are critical for tumor immunity.

Free Research Field

医歯薬学