2014 Fiscal Year Final Research Report
Synaptic integration in mesoscopic circuits in the cerebral cortex
| Project Area | Mesoscopic neurocircuitry: towards understanding of the functional and structural basis of brain information processing |
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| Project/Area Number |
22115005
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
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| Project Period (FY) |
2010-04-01 – 2015-03-31
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| Keywords | 脳・神経 / 高性能レーザー / 大脳皮質 / 神経細胞 / 運動野 |
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| Outline of Final Research Achievements |
We conducted two-photon calcium imaging of mouse layer 2/3 motor cortex during a self-initiated lever-pull task. In the imaging session after 8-9-day training, head-restrained mice had to pull a lever for approximately 600 ms to receive a water drop. We found two types of task-related cells and clarified their spatial distribution. Next, we conducted two-photon calcium imaging in the motor cortex during 14 training sessions of the self-initiated lever-pull task. In layer 2/3, the accuracy of neuronal ensemble prediction of lever trajectory remained unchanged globally during the training period. However, in layer 5a, the ensemble prediction accuracy steadily improved and one-third of neurons, including subcortical-projecting neurons, evolved to contribute substantially to ensemble prediction in the late stage of learning. Furthermore, we performed two-photon imaging of dendritic activity and axonal activity in layer 1 during the mouse task performance.
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| Free Research Field |
神経科学
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