2014 Fiscal Year Final Research Report

Metabolomic analyses of omega-3 fatty acid metabolism and their physiological functions

Planned Research

Project Area	Machineries of bioactive lipids in homeostasis and diseases
Project/Area Number	22116006
Research Category	Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Allocation Type	Single-year Grants
Review Section	Biological Sciences
Research Institution	The Institute of Physical and Chemical Research (2014) The University of Tokyo (2010-2013)
Principal Investigator	ARITA Makoto 独立行政法人理化学研究所, 統合生命医科学研究センター, チームリーダー (80292952)
Project Period (FY)	2010-04-01 – 2015-03-31
Keywords	生理活性脂質 / 脂肪酸代謝 / 抗炎症作用 / メタボロミクス
Outline of Final Research Achievements	We established LC-MS/MS-based lipidomics system to monitor more than 500 of fatty acid metabolites simultaneously. From EPA-lipidomics, we identified potent anti-inflammatory metabolites, namely 17,18-diHEPE and 12-OH-17,18-EpETE, that belong to unique omega-3 PUFA metabolic pathway. By using omega-3 desaturase (fat-1) transgenic mouse, we identified cardioprotective function of macrophage-derived EPA metabolite 18-HEPE. Also we found dysregulated synthesis of omega-3 PUFA-derived anti-inflammatory mediator protectin D1 in eosinophils from patients with severe asthma.
Free Research Field	生物系薬学