2014 Fiscal Year Final Research Report
Temporal and Spacial Analyses of Sensing and Responses to Temperature during Cold Acclimation and Deacclimation in Plants
| Project Area | Environmental sensing of plants: Signal perception, processing and cellular responses |
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| Project/Area Number |
22120003
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Iwate University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWAMURA Yukio 岩手大学, 農学部, 准教授 (10400186)
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| Research Collaborator |
TOMINAGA Yoko
AHAMED Arifa
TAKAHASHI Daisuke
IMAI Hiroyuki
MIKI Yushi
HIRAKI Hayato
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| Project Period (FY) |
2010-04-01 – 2015-03-31
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| Keywords | 低温馴化 / 凍結耐性 / 環境刺激応答 / 細胞膜 / プロテオーム / 遺伝子発現 / 凍結ストレス |
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| Outline of Final Research Achievements |
In this project, we aimed to elucidate molecular mechanisms of cold response in plants to develop cold tolerance, from cold perception to cellular responses. The results revealed that 1) cold acclimation was affected by not only temperature but also light quality, 2) a newly developed in planta monitoring system of gene expression (using a transgenic plant containing a construct of the promoter of the interested gene combined with luciferase gene) was powerful to analyze temporal and special expression patterns of cold-regulated gene during cold acclimation, 3) behavior of ER and Ca in response to cold or freezing could be monitored using unique system of laser scanning confocal microscope and temperature-controlled sample stage, and 4) a cold-induced GPI-anchored protein associated with the plasma membrane was functionally associated with the increase of freezing tolerance. With these results, we discussed the mechanism of temperature perception and signal transduction in plants.
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| Free Research Field |
植物分子生理学
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