Planned Research
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Cell and tissue-specific gene expression profiles are important for understanding how multicellular organisms function and develop. We report a novel, highly reliable position-specific gene expression profiling method developed for plants. It consists of an automatically dissecting system with a narrow, sharpened needle coupled with an optimized protocol to synthesize a high-quality cDNA library on magnetic beads using a single micro-sample. Using this method, gene expression patterning and clustering at successive 50-μm intervals was demonstrated in the shoot apex of a 4-day-old Arabidopsis seedling.
ＤＮＡ等生体関連物質計測
