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2014 Fiscal Year Final Research Report

Mechanisms underlying determination of positional information through Dachsous/Fat signaling system

Planned Research

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Project AreaMolecular mechanisms underlying reconstruction of 3D structers during regeneration
Project/Area Number 22124003
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokushima

Principal Investigator

NOJI Sumihare  徳島大学, 本部, 理事 (40156211)

Co-Investigator(Kenkyū-buntansha) OHUCHI Hideyo  岡山大学, 医歯(薬)学総合研究科, 教授 (00253229)
MITO Taro  徳島大学, 大学院ソシオテクノサイエンス研究部, 助教 (80322254)
BANDO Tetsuya  岡山大学, 医歯(薬)学総合研究科, 助教 (60423422)
Project Period (FY) 2010-04-01 – 2015-03-31
Keywords脚再生 / Dachsous/Fat / Wnt/BMP/EGF / dachshund/Distal-less / マクロファージ / Enhancer of zeste/Utx / ゲノム編集 / ノックイン
Outline of Final Research Achievements

We have investigated molecular mechanisms underlying insect leg regeneration by means of regeneration-dependent RNA interference. Our results suggested that leg regeneration occurs as follows:
(1) Activation of macrophage-like cells by amputation at the amputated site, (2) Cytokines produced by the macrophage-like cells induce formation of a blastema consisting of undifferentiated cells, (3) In the blastema, EGF (epidermal growth factor) is induced by cross-talk of Wnt/BMP signaling pathways. Furthermore, genes involved in epigenetic regulations such as Enhancer of Zest and Utx are activated, (4) EGF induces expressions of dachshund/Distal-less and regulates expression of Dachsous/Fat signaling systems, which then determine re-patterning of the leg and its size. We speculated that the leg size regulation may be related to the number of Dachsous/Fat molecules in each cell membrane with a proximodistal gradient. This was simulated with our modified steepness model.

Free Research Field

再生生物学

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Published: 2016-06-03  

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