Molecular analysis of mating-type switching in fission yeast.

2014 Fiscal Year Final Research Report

• Project Area: Systematic study of chromosome adaptation
• Project/Area Number: 22125003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: IWASAKI Hiroshi 東京工業大学, 生命理工学研究科, 教授 (60232659)
• Research Collaborator: TSUTSUI Yasuhiro
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: 接合型変換 / ヘテロクロマチン / 相同組換え / DNA二重鎖切断 / 分裂酵母 / Swi2-Swi5複合体 / Rad51 / ゲノムアダプテーション

Outline of Final Research Achievements

A fundamental reaction step of fission yeast mating-type (MT) switching is a gene conversion that is driven with a strict polarity. This polarity control is closely related to heterochromatin formation and genetic imprinting at the mat locus. In the research project, we aimed to understand molecular mechanism of MT switching mainly by analyzing chromosome dynamics at the mat locus. ChIP-Seq analysis has revealed that Swi2 localizations at mat locus are uniquely different between two different-MT cells. In addition, 3C analysis has also indicated that chromosome configurations are different in the two different-MT cells.

Free Research Field

分子生物学