Establishment of genome analyses methods for non-model organisms

2015 Fiscal Year Final Research Report

• Project Area: Genetic bases for the evolution of complex adaptive traits
• Project/Area Number: 22128008
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kanazawa University
• Principal Investigator: Nishiyama Tomoaki 金沢大学, 学際科学実験センター, 助教 (50390688)
• Co-Investigator(Kenkyū-buntansha): SHIGENOBU Shuji 基礎生物学研究所, 生物機能解析センター, 特任准教授 (30399555) ; KADOTA Koji 東京大学, 大学院農学生命科学研究科, 特任准教授 (60392221)
• Co-Investigator(Renkei-kenkyūsha): KASAHARA Masahiro 東京大学, 新領域創成科学研究科, 講師 (60376605)
• Research Collaborator: YAMAGUCHI Katsushi
• Project Period (FY): 2010-04-01 – 2016-03-31
• Keywords: アセンブリー / ゲノム / トランスクリプトーム / 遺伝子発現比較

Outline of Final Research Achievements

Genome and transcriptome sequencing, assembly, and annotation methods and expression profile analysis methods were developed to facilitate identification of genes responsible for complex adaptive traits in non-model organisms. Long mate-pair library construction enabled over Mb scaffolds and assembly utilizing long reads with single molecule real-time sequencing technology by Pacificbiosciences enabled over 100-kb contigs in multiple species. A novel robust normalization method for digital gene expression analyses were implemented and distributed in the R package TCC.

Free Research Field

ゲノム進化学