Analysis of novel molecular targets of cancer stem cells by systems analysis

2014 Fiscal Year Final Research Report

• Project Area: Development of Novel Treatment Strategies Targeting Cancer Stem Cells
• Project/Area Number: 22130009
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kanazawa University (2014); The University of Tokyo (2010-2013)
• Principal Investigator: GOTOH Noriko 金沢大学, がん進展制御研究所, 教授 (10251448)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: がん幹細胞 / 受容体 / チロシンキナーゼ / 乳がん / 増殖因子 / アダプター

Outline of Final Research Achievements

In order to analyze HER2/ErbB2-PI3K-NFkB pathway in breast cancer stem cells, DNA microarray analysis was performed by using breast cancer cells stimulated with HER2 in the presence or absence of NFkB inhibitor or PI3K inhibitor. We identified several candidate molecular targets in breast cancer stem cells, IGF2, AR, GDF15, for example. We confirmed that these molecules play important roles for stemness of breast cancer cells.
We analyzed FRS2beta knockout mice in the background of MMTV-ErbB2 mammary tumor model. We found that FRS2beta play important roles for stemness of mammary tumors through fine-tuning of ErbB2 signaling.

Free Research Field

腫瘍生物学