2014 Fiscal Year Final Research Report
Generality of translesion synthesis coupling replication and repair
| Project Area | Coupling of replication, repair and transcription, and their common mechanism of chromatin remodeling |
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| Project/Area Number |
22131008
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Gakushuin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MASUTANI Chikahide 名古屋大学, 環境医学研究科, 教授 (40241252)
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOI Masayuki 学習院大学, 理学部, 助教 (00322701)
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| Project Period (FY) |
2010-04-01 – 2015-03-31
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| Keywords | 損傷乗り越え合成 / 紫外線 / 色素性乾皮症 / DNAポリメラーゼ / PCNA / ユビキチン / 損傷トレランス / X線結晶構造解析 |
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| Outline of Final Research Achievements |
In order to examine the generality of translesion synthesis (TLS), which coordinates replication and repair, we analyzed the structure of co-crystals of human DNA polymerase eta (Pol eta) and CPD-containing DNA with X-ray diffraction. We found that human Pol eta acts like a molecular splint to stabilize damaged DNA in a normal B-form conformation. On the other hand, the crystal structure of human Pol eta and cisplatin-containing DNA revealed that Pol eta could not act like a molecular splint. Mono- or polyubiquitination of PCNA controls the pathway choice of DNA damage tolerance. We demonstrated that PCNA is poly-ubiquitinated via transfer of a pre-formed ubiquitin chain on monoubiquitinated PCNA.
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| Free Research Field |
分子生物学
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