Transcription-coupled repair and protein remodeling

2014 Fiscal Year Final Research Report

• Project Area: Coupling of replication, repair and transcription, and their common mechanism of chromatin remodeling
• Project/Area Number: 22131009
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: TANAKA Kiyoji 大阪大学, 生命機能研究科, 特任教授 (80144450)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: ヌクレオチド除去修復 / 転写と共役した修復 / 色素性乾皮症 / コケイン症候群 / 紫外線高感受性症候群 / ユビキチン化 / SUMO化 / 初期応答遺伝子

Outline of Final Research Achievements

We examined molecular mechanism of transcription-coupled nucleotide excision repair (TC-NER) that quickly removes transcription-blocking DNA damage on the transcribed strands, and leads to resumption of transcription after DNA damage. We found that (1) Ubiquitylation of the largest subunit of RNA polymerase II by the CSA complex is indispensable for TC-NER. (2) Novel TC-NER factor, UVSSA-USP7 complex, is required for protection of CSB from proteasome-mediated degradation after UV-irradiation, leading to resumption of transcription. (3) We revealed novel functions of NER factors, XPG and XPD. XPG is required for transcription elongation of primary response gene FOS and house keeping gene EEF1A1, and XPD is included in the novel protein complex, named as MMXD, which is involved in chromosome segregation.

Free Research Field

分子生物学、人類遺伝学