HLA genome diversity analyses by resequencing

2014 Fiscal Year Final Research Report

• Project Area: HLA polymorphism, disease and evolution
• Project/Area Number: 22133002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokai University
• Principal Investigator: SHIINA Takashi 東海大学, 医学部, 准教授 (00317744)
• Co-Investigator(Kenkyū-buntansha): OTA Masao 信州大学, 医学部, 准教授 (50115333) ; HOSOMICHI Kazuyoshi 金沢大学, 医学系, 准教授 (50420948) ; IMANISHI Tadashi 東海大学, 医学部, 教授 (80270461) ; OHTSHUKA Masato 東海大学, 医学部, 准教授 (90372945)
• Research Collaborator: INOKO Hidetoshi 東海大学, 医学部, 名誉教授
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: HLA / 次世代シークエンサー / ゲノム多様性 / ゲノタイピング / 疾患感受性

Outline of Final Research Achievements

Efficient resequencing of the entire 3.8 Mb HLA region was performed by the long-range PCR and sequence capture methods followed by next generation sequencing. The HLA sequences derived from seven HLA haplotypes were characterized among the different HLA haplotypes and also among the same HLA haplotypes. A field 4 level super high resolution single molecule - sequence based typing (SS-SBT) method was developed for HLA 11 loci using next generation sequencers. Polymorphism analysis on some HLA associated diseases such as Narcolepsy, AIP and PBD were performed at the field 4 level without phase ambiguity by the SS-SBT method. An HLA integrated database (HLA Information Resource; HLA-IR) was constructed as a public database. They will also contribute to the foundation for a refined knowledge of variation and its haplotype structure throughout the extremely polymorphic and gene-rich HLA maps, providing important information on the process of molecular evolution in the HLA region.

Free Research Field

免疫遺伝学