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2014 Fiscal Year Final Research Report

HLA genome diversity analyses by resequencing

Planned Research

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Project AreaHLA polymorphism, disease and evolution
Project/Area Number 22133002
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTokai University

Principal Investigator

SHIINA Takashi  東海大学, 医学部, 准教授 (00317744)

Co-Investigator(Kenkyū-buntansha) OTA Masao  信州大学, 医学部, 准教授 (50115333)
HOSOMICHI Kazuyoshi  金沢大学, 医学系, 准教授 (50420948)
IMANISHI Tadashi  東海大学, 医学部, 教授 (80270461)
OHTSHUKA Masato  東海大学, 医学部, 准教授 (90372945)
Research Collaborator INOKO Hidetoshi  東海大学, 医学部, 名誉教授
Project Period (FY) 2010-04-01 – 2015-03-31
KeywordsHLA / 次世代シークエンサー / ゲノム多様性 / ゲノタイピング / 疾患感受性
Outline of Final Research Achievements

Efficient resequencing of the entire 3.8 Mb HLA region was performed by the long-range PCR and sequence capture methods followed by next generation sequencing. The HLA sequences derived from seven HLA haplotypes were characterized among the different HLA haplotypes and also among the same HLA haplotypes. A field 4 level super high resolution single molecule - sequence based typing (SS-SBT) method was developed for HLA 11 loci using next generation sequencers. Polymorphism analysis on some HLA associated diseases such as Narcolepsy, AIP and PBD were performed at the field 4 level without phase ambiguity by the SS-SBT method. An HLA integrated database (HLA Information Resource; HLA-IR) was constructed as a public database. They will also contribute to the foundation for a refined knowledge of variation and its haplotype structure throughout the extremely polymorphic and gene-rich HLA maps, providing important information on the process of molecular evolution in the HLA region.

Free Research Field

免疫遺伝学

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Published: 2016-06-03  

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