Understanding cancer cell metabolism and biomarker discovery using metabolome analysis

2014 Fiscal Year Final Research Report

• Project Area: Integrative Systems Understanding of Cancer for Advanced Diagnosis, Therapy and Prevention
• Project/Area Number: 22134007
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Keio University
• Principal Investigator: SOGA Tomoyoshi 慶應義塾大学, 環境情報学部, 教授 (60338217)
• Co-Investigator(Renkei-kenkyūsha): SUGIMOTO Masahiro 慶應義塾大学, 政策メディア研究科および先端生命科学研究所, 特任准教授 (30458963) ; HIRAYAMA Akiyoshi 慶應義塾大学, 政策メディア研究科および先端生命科学研究所, 特任助教 (00572405) ; SATO Kiyotoshi 慶應義塾大学, 政策メディア研究科および先端命科学研究所, 特任助教 (50401386) ; TABATA Sho 慶應義塾大学, 政策メディア研究科および先端命科学研究所, 特任助教 (30708342)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: 癌 / 生体分子 / バイオテクノロジー

Outline of Final Research Achievements

Although cancer cells produce ATP and the precursors of macromolecules by switching metabolism, underlying mechanisms have been poorly understood. Here, we have developed a highly sensitive metabolome analysis method based on sheathless CE-MS device.
This approach and together with systems biological technologies such as transcriptomics and proteomics were applied to fumarate hydratase (FH)　knock out mouse and FH deficient cell lines, which mimic Hereditary leiomyomatosis and renal cell carcinoma that develops benign cutaneous and uterine leiomyomas, renal cysts and kidney cancer.
Consequently, we found that accumulated fumarate caused by FH deletion regulated metabolism by activation transcription factor, inactivation of metabolic enzymes and conjugation to metabolites.

Free Research Field

分析化学、メタボロミクス、がんの代謝研究