心筋細胞シグナルパスウェイ制御機構と神経性調節の統合的解明

2014 Fiscal Year Annual Research Report

• Project Area: Establishment of Integrative Multi-level Systems Biology and its Applications
• Project/Area Number: 22136009
• Research Institution: Yokohama City University
• Principal Investigator: 石川 義弘 横浜市立大学, 医学(系)研究科(研究院), 教授 (40305470)
• Co-Investigator(Kenkyū-buntansha): 赤羽 悟美 東邦大学, 医学部, 教授 (00184185) ; 奥村 敏 鶴見大学, 歯学部, 教授 (60233475)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: シグナル伝達 / 生理学 / 循環器・高血圧

Outline of Annual Research Achievements

ｃＡＭＰを介した自律神経シグナルは心機能を調節するだけでなく、Ca2+チャネルの活動を制御して、不整脈発生にも大きな役割を果たす。本研究では、心筋細胞における両者の破綻メカニズムを、不整脈の発生制御の観点から統合的に理解し、抗不整脈剤等の効果を含めた予測医学への発展を目指した。これまでに多階層的システムバイオロジー研究のモデルとして、Ca2+チャネルとアデニル酸シクラーゼを設定し、時間・空間的にどのような複合的な制御を受けるのかを検討した。さらに得られた遺伝子操作動物や作動薬を用いた実験結果と統合することにより、システムバイオロジー手法を用いて、Ca2+とcAMPシグナルの相互抑制作用のメカニズムを検証し、両諸条件を解明した。最終年度である平成２６年度には、これまでの研究成果を統合し、cAMPを介した自律神経シグナルとCa2+チャネルの活動の制御および不整脈研究に向けて実用化に向けた検討を総括し、研究論文として発表した。
すでにCa2+流入によるアデニル酸シクラーゼの直接抑制だけでは、長期的な時間的位相差を示すオシレーションは困難であり、リン酸化や自律神経の活性化による刺激を組み入れることが、位相差の維には必要である。これらの条件をCell Designerを用いて検証し、細胞内Ca2+濃度がｃＡＭＰ濃度とオシレーションする諸条件を時間的に解明した。さらに数理モデルを動物モデルで検証するために、心臓型アデニル酸シクラーゼ欠損動物でｃＡＭＰシグナルの質的な変化をもたせ、心房性を中心に不整脈の誘導性を検討した。Cell　Designerとの連動性の検討によって、生体制御系の諸因子を取り込みながら、さらに同一班内の研究者と検討し、不整脈を中心とする心疾患における予測および治療標的の検討を進めることができた。

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Oscillation of cAMP and Ca2+ in cardiac myocytes. (2015)
  • Author(s): Kamide T, Okumura S, Ghosh S, Shinoda Y, Mototani Y, Ohnuki Y, Jin H, Cai W, Suita K, Sato I, Umemura M, Fujita T, Yokoyama U, Sato M, Frutani K, Kitano H, and Ishikawa Y.
  • Journal Title: J. Physiol. Sci. Volume: 65 Pages: 195-200
  • DOI: 10.1007/s12576-014-0354-3. Epub 2015 Jan 14.
  • Peer Reviewed
• [Journal Article] Coupling of beta1-adrenergic receptor to type 5 adenylyl cyclase and its physiological relevance in cardiac myocytes. (2015)
  • Author(s): Tsunematsu T, Okumura S, Mototani Y, Ohnuki Y, Jin H, Cai W, SuitaK, Sato I, Umemura M, Yokoyama U, Sato M, Fujita T, and Ishikawa Y.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 458 Pages: 531-535
  • DOI: 10.1016/j.bbrc.2015.01.149. Epub 2015 Feb 9.
  • Peer Reviewed
• [Journal Article] A magnetic anti-cancer compound for magnet-guided delivery and magnetic resonance imaging. (2015)
  • Author(s): Eguchi H, Umemura M, Kurotani R, Fukumura H, Sato I, Kim J-H, Hoshino Y, Lee J, Amemiya N, Sato M, Hirata K, Sigh DJ, Masuda T, Yamamoto M, Urano T, Yoshida K, Tanigaki K, Yamamoto M, Sato M, Inoue S, Aoki I and Ishikawa Y.
  • Journal Title: Sci. Rep. Volume: 5 Pages: 9194
  • DOI: 10.1038/srep09194.
  • Peer Reviewed
• [Journal Article] Prostaglandin E2 inhibits elastogenesis in the ductus arteriosus via EP4 signaling. (2014)
  • Author(s): Yokoyama U, Minamisawa S, Shioda A, Ishiwata R, Jin MH, Masuda M, Asou T, Sugimoto Y, Aoki H, Nakamura T, and Ishikawa Y
  • Journal Title: Circulation. Volume: 129 Pages: 487-496
  • DOI: 10.1161/CIRCULATIONAHA.113.004726. Epub 2013 Oct 21.
  • Peer Reviewed
• [Journal Article] Store-operated Ca²⁺ entry (SOCE) Regulates Melanoma Proliferation and Cell Migration. Cell (2014)
  • Author(s): Umemura M, Baljinnyam E, Feske S, De Lorenzo MS, Xie LH, Feng X, Oda K, Makino A, Fujita T, Yokoyama U, Iwatsubo M, Chen S, Goydos JS, Ishikawa Y, and Iwatsubo K
  • Journal Title: PloS One. Volume: 9 Pages: e89292
  • DOI: 10.1371/journal.pone.0089292. eCollection 2014.
  • Peer Reviewed
• [Journal Article] Hyperthermia generated with ferucarbotran (Resovist®) in an alternating magnetic field enhances cisplatin-induced apoptosis of cultured human oral cancer cells. (2014)
  • Author(s): Sato I, Umemura M, Mitsudo K, Kioi M, Nakashima H, Iwai T, Feng X, Oda K, Miyajima A, Makino A, Iwai M, Fujita T, Yokoyama U, Okumura S, Sato M, Eguchi H, Tohnai I, Ishikawa Y
  • Journal Title: J Physiol Sci. Volume: 64 Pages: 177-183
  • DOI: 10.1007/s12576-014-0309-8. Epub 2014 Mar 12.
  • Peer Reviewed
• [Journal Article] Decreased serum osmolality promotes ductus arteriosus constriction. (2014)
  • Author(s): Aoki R, Yokoyama U, Ichikawa Y, Taguri M, Kumagaya S, Ishiwata R, Yanai C, Fujita S, Umemura M, Fujita T, Okumura S, Sato M, Minamisawa S, Asou T, Masuda M, Iwasaki S, Nishimaki S, Seki K, Yokota S, and Ishikawa Y
  • Journal Title: Cardiovasc. Res. Volume: 104 Pages: 326-336
  • DOI: 10.1093/cvr/cvu199. Epub 2014 Sep 4.
  • Peer Reviewed
• [Journal Article] Three-dimensional multilayers of smooth muscle cells as a new experimental model for vascular elastic fiber formation studies. (2014)
  • Author(s): Ishiwata R, Yokoyama U, Matsusaki M, Asano Y, Kadowaki K, Ichikawa Y, Umemura M, Fujita T, Minamisawa S, Shimoda H, Akashi M, Ishikawa Y
  • Journal Title: Atherosclerosis. Volume: 233 Pages: 590-600
  • DOI: 10.1016/j.atherosclerosis.2014.01.045. Epub 2014 Jan 29.
  • Peer Reviewed
• [Journal Article] Protection of cardiomyocytes from the hypoxia-mediated injury by a Peptide targeting the activator of g-protein signaling 8. (2014)
  • Author(s): Sato M, Hiraoka M, Suzuki H, Sakima M, Mamun AA, Yamane Y, Fujita T, Yokoyama U, Okumura S, Ishikawa Y
  • Journal Title: PLoS One. Volume: 9 Pages: e91980
  • DOI: 10.1371/journal.pone.0091980. eCollection 2014.
  • Peer Reviewed
• [Journal Article] Epac1-dependent phospholamban phosphorylation mediates the cardiac response to stresses. (2014)
  • Author(s): Okumura S, Fujita T, Cai W, Jin M, Namekata I, Mototani Y, Jin H, Ohnuki Y, Tsuneoka Y, Kurotani R, Suita K, Kawakami Y, Hamaguchi S, Abe T, Kiyonari H, Tsunematsu T, Bai Y, Suzuki S, Hidaka Y, Umemura M, Ichikawa Y, Yokoyama U, Sato M, Ishikawa F, Izumi-Nakaseko H, Adachi-Akahane S, Tanaka H, Ishikawa Y
  • Journal Title: J Clin Invest. Volume: 124 Pages: 2785-2801
  • DOI: 10.1172/JCI64784. Epub 2014 Apr 24.
  • Peer Reviewed
• [Journal Article] Exendin-4 ameliorates cardiac ischemia/reperfusion injury via caveolae and caveolins-3. (2014)
  • Author(s): Tsutsumi YM, Tsutsumi R, Hamaguchi E, Sakai Y, Kasai A, Ishikawa Y, Yokoyama U, and Tanaka K.
  • Journal Title: Cardiovasc Diabetol. Volume: 13 Pages: 132
  • DOI: 10.1186/s12933-014-0132-9.
  • Peer Reviewed
• [Journal Article] Geranylgeranylacetone protects the heart via caveolae and caveolin-3. (2014)
  • Author(s): Tsutsumi YM, Tsutsumi R, Hirokawa YT, Sakai Y, Hamaguchi E, Ishikawa Y, Yokoyama U, Kasai A, Kambe N, and Tanaka K.
  • Journal Title: Life Sciences. Volume: 101 Pages: 43-48
  • DOI: 10.1016/j.lfs.2014.02.019. Epub 2014 Feb 26.
  • Peer Reviewed
• [Journal Article] Epac1 increases migration of endothelial cells and melanoma cells via FGF2-mediated paracrine signaling. (2014)
  • Author(s): Baljinnyam E, Umemura M, Chuang C, De Lorenzo M, Iwatsubo M, Chen S, Goydos J, Ishikawa Y, Whitelock J, and Iwatsubo K
  • Journal Title: Pigment Cell & Melanoma Research. Volume: 27 Pages: 611-620
  • DOI: 10.1111/pcmr.12250. Epub 2014 May 9.
  • Peer Reviewed
• [Journal Article] A Case of R-II-B Type Single Coronary Artery Evaluated by Multi-Detector Computed Tomography and Coronary Angiography. (2014)
  • Author(s): Narikawa M, Kiyokuni M, Umemura M, Kawashima C, Doi H, Hisa A, Tomari S, Mitsuhashi T, Ishikawa Y and Endo T.
  • Journal Title: J Clin Exp Cardiolog, Volume: 5 Pages: 10
  • Peer Reviewed
• [Journal Article] Impaired NO Production and Increased Blood Pressure in Systemic Heterozygous ATP2B1 Null Mice. (2014)
  • Author(s): Fujiwara A, Hirawa N, Fujita M, Kobayashi Y, Okuyama Y, Yatsu K, Katsumata M, Yamamoto y, Ichihara N, Saka S, Toya Y, Yasuda G, Tabara Y, Miki T, Ueshima H, Ishikawa Y, and Umemura S
  • Journal Title: J Hypertension. Volume: 32 Pages: 1415-1423
  • DOI: 10.1097/HJH.0000000000000206.PMID:24805951
  • Peer Reviewed
• [Journal Article] Deletion of the angiotensin II type 1 receptor-associated protein enhances renal sodium reabsorption and exacerbates angiotensin II-mediated hypertension. (2014)
  • Author(s): Ohsawa M, Tamura K, Wakui H, Maeda A, Dejima T, Kanaoka T, Azushima K, Uneda K, Tsurumi-Ikeya Y, Kobayashi R, Matsuda M, Uchida S, Toya Y, Kobori H, Nishiyama A, Yamashita A, Ishikawa Y, and Umemura S
  • Journal Title: Kidney International. Volume: 86 Pages: 570-581
  • DOI: 10.1038/ki.2014.95. Epub 2014 Apr 2.
  • Peer Reviewed
• [Journal Article] Role of Cyclic AMP Sensor Epac1 in Masseter Muscle Hypertrophy and Myosin Heavy Chain Transition Induced by 2-Adrenoceptor Stimulation. (2014)
  • Author(s): Ohnuki Y, Umeki D, Mototani Y, Jin H, Cai W, Shiozawa K, Suita K, Saeki Y, Fujita T, Ishikawa Y, and Okumura S.
  • Journal Title: J. Physiol. Volume: 592 Pages: 5461-5475
  • DOI: 10.1113/jphysiol.2014.282996. Epub 2014 Oct 24.
  • Peer Reviewed
• [Journal Article] Obstructive Sleep Apnea and Hypertension:A New Way to Go. (2014)
  • Author(s): Ishikawa Y.
  • Journal Title: J Sleep Disorders Ther. Volume: 3 Pages: 4
  • Peer Reviewed
• [Presentation] Cardiac overexpression of Translationally Controlled Tumor Protein (TCTP) prevents the development of Dihydroartemisinin (DHA)-induced heart. (2015)
  • Author(s): Cai W, Fujita T, Hidaka Y, Jin H, Hasegawa N, Ishikawa Y.
  • Organizer: 第79回日本循環器学会学術集会
  • Place of Presentation: 大阪国際会議場、リーガロイヤルホテル大阪、堂島リバーフォーラム、ＡＢＣホール(大阪府）
  • Year and Date: 2015-04-24 – 2015-04-26
• [Presentation] Transient receptor potential cation channel 3 (TRPC3) regulates tumor proliferation and migration of BRAF wild type human malignant melanoma. (2015)
  • Author(s): Oda K, Umemura M, Baljinntam E, Yamaguchi Y, Aihara M, Iwatsubo K, Ishikawa Y.
  • Organizer: The 106th American Association for Cancer Research Annual meeting.
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2015-04-18 – 2015-04-22
• [Presentation] Methotrexate derivative with intrinsic magnetism. (2015)
  • Author(s): Umemura M, Katsumata M, Sato I, Nagasako A, Aoyama H, Makino A, Ohatake M, Oda K, Eguchi H, Ishikawa Y.
  • Organizer: The 106th American Association for Cancer Research Annual meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2015-04-18 – 2015-04-22
• [Presentation] Simultaneous hyperthermic-chemotherapy for glioblastoma using a single anti-cancer compound with magnetism. (2015)
  • Author(s): Ohtake M, Umemura M, Sato I, Oda K, Nagasako A, Makino A, Aoyama H, Katsumata M, Eguchi H, Kawahara N , Ishikawa Y.
  • Organizer: The 106th American Association for Cancer Research Annual meeting.
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2015-04-18 – 2015-04-22
• [Presentation] A novel methotrexate derivative with intrinsic magnetism. (2015)
  • Author(s): Katsumata M, Umemura M, Sato I, Ohtake M, Oda K, Nagasako A, Makino A, Aoyama H, Eguchi H, Ishikawa Y.
  • Organizer: 第92回日本生理学会大会
  • Place of Presentation: 神戸国際会議場・展示場（兵庫県）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Magnetized paclitaxel derivative for novel triple negative breast cancer therapy. (2015)
  • Author(s): Makino A, Umemura M, Sato I, Katsumata M, Nagasako A, Oda K, Ohtake M, Aoyama H, Eguchi H, Ishikawa Y.
  • Organizer: 第92回日本生理学会大会
  • Place of Presentation: 神戸国際会議場・展示場（兵庫県）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] A novel methotrexate derivative with intrinsic magnetism. (2015)
  • Author(s): Katsumata M, Umemura M, Sato I, Ohtake M, Oda K, Nagasako A, Makino A, Aoyama H, Eguchi H, Ishikawa Y.
  • Organizer: 第88回日本薬理学会
  • Place of Presentation: 名古屋国際会議場（愛知県）
  • Year and Date: 2015-03-18 – 2015-03-20
• [Presentation] プロスタグランディンE－EP4はfibulin-1を介して生理的・病的血管内膜肥厚の形成に関与する． (2014)
  • Author(s): 矢内千春, 横山詩子, 熊谷 駿, 手島隆之, 佐藤綾美, 石川義弘
  • Organizer: 第13回日本心臓血管発生研究会
  • Place of Presentation: 磐梯熱海温泉 ホテル 華の湯(福島県）
  • Year and Date: 2014-10-17 – 2014-10-18
• [Presentation] 抗ヘルペス薬による心房細動抑制． (2014)
  • Author(s): 吹田憲治, 藤田孝之, 奥村 敏, 石川義弘
  • Organizer: 第131回日本薬理学会関東部会
  • Place of Presentation: 横浜市立大学医学部（神奈川県）
  • Year and Date: 2014-10-11 – 2014-10-11
• [Presentation] A novel paclitaxel with intrinsic magnetism. (2014)
  • Author(s): Makino A, Umemura M, Sato I, Oda K, Otake M, Nagasako A, Eguchi H, Ishikawa Y.
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Development of combination therapy with cisplatin and hyperthermia generated with ferucarbotran (Resovist) in an alternating magnetic field for oral cancer. (2014)
  • Author(s): Sato I, Mitsudo K, Umemura M, Nakashima H, Kioi M, Eguchi H, Ishikawa Y,Tohnai.I
  • Organizer: The 6th Asian Congress of Hyperthermic Oncology (ACHO)
  • Place of Presentation: 福井県民ホール・福井市地域交流プラザ（福井県）
  • Year and Date: 2014-09-05 – 2014-09-06
• [Presentation] EPAC1 plays an important role in regulation of renin and AQP2 expression in the kidney of mice. (2014)
  • Author(s): Jin H, Fujita T, Cai W, Hidaka Y, Sagara S, Kubota K, Suita K, Ishikawa Y.
  • Organizer: European Society of Cardiorogy Congress.
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2014-08-30 – 2014-09-03
• [Presentation] Prostaglandin E2-EP4 plays a protective role against cardiac fibrosis. (2014)
  • Author(s): Ishiwata R, Yokoyama U, Inoue S, Ichikawa Y, Ishikawa Y.
  • Organizer: European Society of Cardiorogy Congress.
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2014-08-30 – 2014-09-03
• [Presentation] Cardiac overexpression ofure. Chronic Treatment with Dihydroartemisinin, a TCTP Down-regulating Agent, Results in Cardiac Dysfunction in Mice． (2014)
  • Author(s): Cai W, Fujita T, Hidaka Y, Jin H, K. Sagara S, Kubota K, Suita K, Ishikawa Y.
  • Organizer: European Society of Cardiorogy Congress.
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2014-08-30 – 2014-09-03
• [Presentation] Vidarabine, an anti-herpesvirus agent, prevents catecholamine-induced atrial fibrillation in mice. (2014)
  • Author(s): Sujita K, Fujita T, Cai W, Hidaka Y, Jin H, Jin M, Okumura S, Ishikawa Y.
  • Organizer: European Society of Cardiorogy Congress.
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2014-08-30 – 2014-09-03
• [Presentation] 体液量調節におけるEPAC1の役割についての検討. (2014)
  • Author(s): Jin H, Fujita T, Cai W, Hidaka Y, Sagara S, Suita K, Ishikawa Y
  • Organizer: 第５７会日本腎臓学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2014-07-04 – 2014-07-06
• [Presentation] ヒト大動脈瘤組織におけるPGE2-EP4誘導性分泌タンパク質の網羅的解析. (2014)
  • Author(s): 石渡 遼, 横山詩子, 荒川憲昭, 安田章沢, 郷田素彦, 鈴木伸一, 益田宗孝, 石川義弘
  • Organizer: 第35回日本循環制医学会総会
  • Place of Presentation: 九州大学医学部百年講堂（福岡県）
  • Year and Date: 2014-07-04 – 2014-07-05
• [Presentation] 心臓型アデニル酸シクラーゼの選択的阻害剤ビダラビンによる心房細動抑制. (2014)
  • Author(s): 吹田憲治, 藤田孝之, 蔡 文倩, 金 慧玲, 奥村 敏, 石川義弘
  • Organizer: 第35回日本循環制御医学会総会
  • Place of Presentation: 九州大学医学部百年講堂（福岡県）
  • Year and Date: 2014-07-04 – 2014-07-05
• [Presentation] MRI造影剤フェルカルボトランを用いた新しい温熱化学療法の開発. (2014)
  • Author(s): 佐藤 格, 光藤健司, 梅村将就, 宮島章嘉, 中島英行, 來生 知, 石川義弘, 藤内 祝.
  • Organizer: 第38回日本頭頚部癌学会
  • Place of Presentation: 東京ファッションタウンビルホール（東京都）
  • Year and Date: 2014-06-12 – 2014-06-13
• [Presentation] Nuclear Factor kappa B Inhibition Promotes Closure of the Rat Ductus Arteriosus. (2014)
  • Author(s): Kajimura I, Yokoyama U, Ishikawa Y, Minamisawa S.
  • Organizer: Experimental Biology Annual Meeting.
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-26 – 2014-04-30
• [Presentation] Prostaglandin E2 receptor EP4 signaling in vascular smooth muscle cells decreases aortic elasticity. (2014)
  • Author(s): Ichikawa Y, Yokoyama U, Ishikawa Y.
  • Organizer: Experimental Biology Annual Meeting.
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-26 – 2014-04-30
• [Presentation] Development of thermochemotherapy using cisplatin and ferucarbotran (Resovist®) in head and neck cancer. (2014)
  • Author(s): Sato I, Umemura M, Mitsudo K, Feng X, Nakashima H, Kioi M, Eguchi H, Tohnai I, Ishikawa Y
  • Organizer: The 105th American Association for Cancer Research Annual meeting.
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-05 – 2014-04-09
• [Presentation] A novel treatment for triple-negative breast cancer using intrinsic magnetized paclitaxel. (2014)
  • Author(s): Umemura M, Makino A, Sato I, Feng X, Iwai M, Oda K, Miyajima A, Otake M, Nagasako A, Matsuo K, Eguchi H, and Ishikawa Y.
  • Organizer: The 105th American Association for Cancer Research Annual meeting.
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-05 – 2014-04-09
• [Book] 標準生理学 第8版 第29章「赤血球」 (2014)
  • Author(s): 石川義弘
  • Total Pages: 1140
  • Publisher: 医学書院
• [Book] 標準生理学 第8版 第39章「局所循環」 (2014)
  • Author(s): 石川義弘
  • Total Pages: 1140
  • Publisher: 医学書院
• [Remarks] 横浜市立大学大学院医学研究科 循環制御医学 ホームページ
  • URL: http://www-user.yokohama-cu.ac.jp/~seiri1/index.html
• [Patent(Industrial Property Rights)] グルタミン酸による動脈管開存症の予防又は治療 (2015)
  • Inventor(s): 横山詩子、石川義弘、藤田秀次郎
  • Industrial Property Rights Holder: 公立大学法人横浜市立大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: PCT/JP2015/054277
  • Filing Date: 2015-02-17
  • Overseas
• [Patent(Industrial Property Rights)] 三次元細胞集合体の作製方法 (2015)
  • Inventor(s): 横山詩子、石川義弘、金子真、佐久間臣耶、新井史人
  • Industrial Property Rights Holder: 横浜市立大学、大阪大学、名古屋大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願 2015-033064
  • Filing Date: 2015-02-23
• [Patent(Industrial Property Rights)] 三次元組織体及びその製造方法 (2014)
  • Inventor(s): 横山詩子、石川義弘、明石満、松崎典弥
  • Industrial Property Rights Holder: 公立大学法人横浜市立大学、国立大学法人大阪大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: PCT/JP2014/77083
  • Filing Date: 2014-10-09
  • Overseas