Development of a bio-screening method using intestinal epithelial cells

2015 Fiscal Year Final Research Report

• Project Area: Chemical Biology using bioactive natural products as specific ligands: identification of molecular targets and regulation of bioactivity
• Project/Area Number: 23102003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Okamoto Ryuichi 東京医科歯科大学, 再生医療研究センター, 教授 (50451935)
• Co-Investigator(Kenkyū-buntansha): Nakamura Tetsuya 東京医科歯科大学, 医歯学(薬)学総合研究科, 寄附講座教授 (70265809)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 腸管上皮 / 初代培養 / 天然物リガンド / 生体活性評価

Outline of Final Research Achievements

Based on our culture method for primary human intestinal cells, we developed methods by which we can visualize and trace stem cells by lentiviral infection, or visualize their daughter cells through genetic modification in mice. Also, we developed our quantitative measurement system to evaluate the fluid/anion transport by intestinal epithelial cells, in addition to the system dedicated to measure the function of P-glycoprotein. Finally, through our collaborative work with professor Ishibashi of A02 group, we established an activity measurement system for Notch-receptor mediated signaling.

Free Research Field

消化器内科