Cell Measurement and Monitoring of Self-directed Induction Using Ultrahigh-speed Manipulation

2015 Fiscal Year Final Research Report

• Project Area: Hyper Bio Assembler for 3D Celluler Innovation
• Project/Area Number: 23106002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Nagoya University
• Principal Investigator: Arai Fumihito 名古屋大学, 工学(系)研究科(研究院), 教授 (90221051)
• Co-Investigator(Kenkyū-buntansha): KAWAHARA Tomohiro 九州工業大学, 若手研究者フロンティア研究アカデミー, 准教授 (20575162) ; MARUYAMA Hisataka 名古屋大学, 大学院工学研究科, 准教授 (60377843)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: マイクロ・ナノデバイス / 超精密計測 / マイクロマシン / バイオ関連機器 / 細胞・組織

Outline of Final Research Achievements

We studied the design and fabrication method of microfluidic chip integrating microactuator and microsensor to measure the multi-parameter of cell. We developed the precise measurement method of probe position with a sub-pixel level of resolution using the phase detection of moire fringe. And we developed the microfluidic chip having multi fluorescence sensor pillars for measuring several physiological parameters such as pH, temperature, and Calcium ions. By using these technologies, we measured the stiffness of cell and spheroid in high-speed, and time-course variation of physiological conditions was measured in the chip. Moreover, we developed rapid fabrication method of multilayer tubular tissue as a model artery, and constructed the perfusion culture environment. By using this model artery and culture system, we found the long-term mechanical stimulus to model artery is effective to form the elastic fiber.

Free Research Field

機械工学