超高速微細操作技術を用いた３次元細胞システム構築

2015 Fiscal Year Annual Research Report

• Project Area: Hyper Bio Assembler for 3D Celluler Innovation
• Project/Area Number: 23106005
• Research Institution: Osaka University
• Principal Investigator: 新井 健生 大阪大学, 基礎工学研究科, 教授 (90301275)
• Co-Investigator(Kenkyū-buntansha): 小嶋 勝 大阪大学, 基礎工学研究科, 助教 (00533647) ; 前 泰志 大阪大学, 基礎工学研究科, 准教授 (50304027) ; 大原 賢一 名城大学, 理工学部, 准教授 (50517886) ; 吉川 洋史 埼玉大学, 理工学研究科, 准教授 (50551173) ; 松井 裕史 筑波大学, 医学医療系, 講師 (70272200)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: マイクロマニピュレーション / 高速3次元計測 / 3次元細胞システムパーツ / 3次元細胞システム / in vitro活性

Outline of Annual Research Achievements

in vitroにおける活性化する3次元細胞システムの構築プロトコルの確立を目指し，本研究では，大きく次の3つの課題を設定して推進した．１．マイクロ流体チップを用いたロボットアームによる3次元細胞システムの生成，２．可変モールドを用いた任意形状3次元細胞パーツの生成及び組み立て，３．高速細胞システム構築技術の開発．本年度は，これまでに確立してきた各課題における生成法の精緻化，方法論の確立を進めると共に細胞を用いた組織構築への適用を行うことを目的とした．
【マイクロ流体チップを用いたロボットアームによる3次元細胞システム生成】マイクロ流体チップを用いた新たな管状細胞パーツ構築法を提案し，その場かん流培養可能な管状構造の構築に成功した．さらに，ロボットアームを組み合わせることによって，自動的に3次元細胞システムを生成するためのシステム設計を行い，肝臓の構造の構築が可能な精度での細胞内包ハイドロゲルファイバの配置に成功した。
【任意形状3次元細胞パーツの生成】マイクロヒータアレイを用いた新規可変モールドシステムを開発し，細胞パーツ形成に応用した．また，マイクロヒータアレイを実装したロボットアームとハイドロゲルと組み合わせることで，複数の細胞パーツの配置法を確立し，任意形状の3次元細胞パーツの構築手法の検証を行った．
【3次元細胞システムの高速構築技術の開発】in vitro環境で活性化する3次元細胞システムの構築において，マイクロハンドの高速動作及び制御手法，3次元細胞パーツと3次元細胞システムの高速検出アルゴリズム，顕微鏡システムのユーザインタフェースを開発し，3次元細胞システムの組み立てへの応用可能性を検証した．

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] University of Heidelberg(Germany)
  • Country Name: Germany
  • Counterpart Institution: University of Heidelberg
• [Journal Article] Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy (2016)
  • Author(s): 4)T. Matsuzaki, K. Ito, K. Masuda, E. Kakinuma, R. Sakamoto, K. Iketaki, H. Yamamoto, M. Suganuma, N. Kobayashi, S. Nakabayashi, T. Tanii, H. Y. Yoshikawa
  • Journal Title: Journal of Physical Chemistry B Volume: 120 Pages: 1221-1227
  • DOI: 10.1021/acs.jpcb.5b11870
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reactive oxygen species induced by non-steroidal anti-inflammatory drugs enhance the effects of photodynamic therapy in gastric cancer cells (2016)
  • Author(s): 5)Hiromu Ito, Hirofumi Matsui, Aki Hirayama, Hiroko P. Indo, Hideyuki J. Majima and Ichinosuke Hyodo
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 58 Pages: 1-6
  • DOI: 10.3164/jcbn.15-124
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Tracking mechanical and morphological dynamics of regenerating Hydra tissue fragments using a two fingered micro-robotic hand (2016)
  • Author(s): M. Veschgini, F. Gebert, N. Khangai, H. Ito, R. Suzuki, T. W. Holstein, Y. Mae, T. Arai, and M. Tanaka
  • Journal Title: Applied Physics Letters Volume: 108 Pages: 103702 1-4
  • DOI: 10.1063/1.4943402
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Development of an optimum end-effector with a nano-scale uneven surface for non-adhesion cell manipulation using a micro-manipulator (2015)
  • Author(s): 1)M Horade, M Kojima, K Kamiyama, T Kurata, Y Mae and T Arai
  • Journal Title: Journal of Micromechanics and Microengineering Volume: 25 Pages: 115002-115010
  • DOI: 10.1088/0960-1317/25/11/115002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Piezo-actuated parallel mechanism for biological cell release at high speed (2015)
  • Author(s): 2)Ebubekir Avci, Takayuki Hattori, Kazuto Kamiyama, Masaru Kojima, Mitsuhiro Horade, Yasushi Mae, Tatsuo Arai
  • Journal Title: Biomedical Microdevices Volume: 17 Pages: 98
  • DOI: 10.1007/s10544-015-0001-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Bacterial sheet-powered rotation of a micro-object (2015)
  • Author(s): 3)Masaru Kojima, Tatsuya Miyamoto, Masahiro Nakajima, Michio Homma, Tatsuo Arai, Toshio Fukuda
  • Journal Title: Sensors and Actuators B: Chemical Volume: 222 Pages: 1220-1225
  • DOI: 10.1016/j.snb.2015.07.071
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Mechanical Manipulation of Adherent Cells by Photodegradable Hydrogel (2015)
  • Author(s): 15.K. Iketaki, F. Yanagawa, R. Kawamura, S. Nakabayashi, T. Takagi, S. Sugiura, T. Kanamori, H. Y. Yoshikawa
  • Organizer: Pacifichem 2015
  • Place of Presentation: Hawaii Convention Center etc., Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15 – 2015-12-20
  • Int'l Joint Research
• [Presentation] Design of Mechanically Patterned Hydrogel for Directing Organ Bud Self-Formation (2015)
  • Author(s): 16.Y. Shimokawa, H. Koike, T. Matsuzaki, M. Enomura, M. Kimura, S. Nakabayashi, H. Nakauchi, H. Taniguchi, T. Takebe, H. Y. Yoshikawa
  • Organizer: Pacifichem 2015
  • Place of Presentation: Hawaii Convention Center etc., Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15 – 2015-12-20
  • Int'l Joint Research
• [Presentation] Automated Construction System for Cellular Tissue Based on Laminar Flow Fibers (2015)
  • Author(s): 11.Masaru Kojima, Mitsuhiro Horade, Akira Fukushima, Kenichi Ohara, Shinji Sakai, Kazuto Kamiyama, Yasushi Mae, Tatsuo Arai
  • Organizer: 2015 International Symposium on Micro-Nano Mechatronics and Human Science
  • Place of Presentation: Nagoya University, Nagoya, Aichi, Japan
  • Year and Date: 2015-11-23 – 2015-11-25
  • Int'l Joint Research
• [Presentation] Development of High Speed Measurement System for Cell-Scaffold Interaction (2015)
  • Author(s): 12.Yu Hirano, Masaru Kojima, Mitsuhiro Horade, Kazuto Kamiyama, Yasushi Mae, Tatsuo Arai
  • Organizer: 2015 International Symposium on Micro-Nano Mechatronics and Human Science
  • Place of Presentation: Nagoya University, Nagoya, Aichi, Japan
  • Year and Date: 2015-11-23 – 2015-11-25
  • Int'l Joint Research
• [Presentation] Automatic stage construction for the support of micro object grasping (2015)
  • Author(s): 13.Kotaro Yamamoto, Masaru Kojima, Mitsuhiro Horade, Kazuto Kamiyama, Yasushi MAE, Tatsuo Arai
  • Organizer: 2015 International Symposium on Micro-Nano Mechatronics and Human Science
  • Place of Presentation: Nagoya University, Nagoya, Aichi, Japan
  • Year and Date: 2015-11-23 – 2015-11-25
  • Int'l Joint Research
• [Presentation] Study on cell manipulation method with high‐accuracy three-dimentional positioning using a manipulator (2015)
  • Author(s): 7.Mitsuhiro Horade, Tomoyuki Kurata, Masaru Kojima, Kazuto Kamiyama, Yasushi Mae and Tatsuo Arai
  • Organizer: 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: Hwabaek International Convention Center (HICO), Gyeongju, Korea
  • Year and Date: 2015-10-25 – 2015-10-29
  • Int'l Joint Research
• [Presentation] Development of novel micro fluidic system without Channels and wells for trapping-and-releasing of micro-objects (2015)
  • Author(s): 8.Mitsuhiro Horade, Masaru Kojima, Kazuto Kamiyama, Yasushi Mae and Tatsuo Arai
  • Organizer: 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: Hwabaek International Convention Center (HICO), Gyeongju, Korea
  • Year and Date: 2015-10-25 – 2015-10-29
  • Int'l Joint Research
• [Presentation] 4D Mciro-structure assembling method by micro-heater array device (2015)
  • Author(s): 9.Suguru Takata, Masaru Kojima, Mitsuhiro Horade, Kazuto Kamiyama, Yasushi Mae and Tatsuo Arai
  • Organizer: 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: Hwabaek International Convention Center (HICO), Gyeongju, Korea
  • Year and Date: 2015-10-25 – 2015-10-29
  • Int'l Joint Research
• [Presentation] Development of gripper on-board micro-heater and force sensor for high-accuracy micro-manipulation (2015)
  • Author(s): 10.Mitsuhiro Horade, Tomoyuki Kurata, Masaru Kojima, Kazuto Kamiyama, Yasushi Mae and Tatsuo Arai
  • Organizer: 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: Hwabaek International Convention Center (HICO), Gyeongju, Korea
  • Year and Date: 2015-10-25 – 2015-10-29
  • Int'l Joint Research
• [Presentation] Generation of Swirl Flow by Needle Vibration for Micro Manipulation (2015)
  • Author(s): 4.Takayuki Hattori, Kazuto Kamiyama, Masaru Kojima, Mitsuhiro Horade, Yasushi Mae, Tatsuo Arai
  • Organizer: 2015 IEEE/RSJ International Conference on Intelligent Robots and Systems
  • Place of Presentation: Congress Center Hamburg (CCH), Hamburg, Germany
  • Year and Date: 2015-09-28 – 2015-10-03
  • Int'l Joint Research
• [Presentation] Development of thermos responsive gel coated end effector for micro manipulation (2015)
  • Author(s): 5.Hideaki Saijo, Masaru Kojima, Mitsuhiro Horade, Kazuto Kamiyama, Yasushi Mae, and Tatsuo Arai
  • Organizer: 2015 IEEE/RSJ International Conference on Intelligent Robots and Systems
  • Place of Presentation: Congress Center Hamburg (CCH), Hamburg, Germany
  • Year and Date: 2015-09-28 – 2015-10-03
  • Int'l Joint Research
• [Presentation] Releasing and Accurate Placing of Adhered Micro-Objects using High Speed motion of End Effector (2015)
  • Author(s): 6.Eunhye Kim, Masaru Kojima, Kazuto Kamiyama, Mitsuhiro Horade, Yasushi Mae, and Tatsuo Arai
  • Organizer: 2015 IEEE/RSJ International Conference on Intelligent Robots and Systems
  • Place of Presentation: Congress Center Hamburg (CCH), Hamburg, Germany
  • Year and Date: 2015-09-28 – 2015-10-03
  • Int'l Joint Research
• [Presentation] Physical Studies of Cell-Substrate Mechanical Interactions for the Regulation of Cell Migration and Tissue Formation (2015)
  • Author(s): H. Y. Yoshikawa
  • Organizer: nternational symposium of Hierarchical Dynamics in Soft Materials and Biological Matter
  • Place of Presentation: iCeMS, Kyoto
  • Year and Date: 2015-09-23 – 2015-09-26
  • Invited
• [Presentation] ハイドロゲルファイバを用いた組織様構造の構築 (2015)
  • Author(s): 嶋勝（阪大）福島英（阪大）洞出光洋（阪大）大原賢一（名城大）神山和人（阪大）前泰志（阪大）新井健生（阪大）
  • Organizer: 第33回日本ロボット学会学術講演会
  • Place of Presentation: 東京電機大学東京千住キャンパス, 東京都足立区
  • Year and Date: 2015-09-03 – 2015-09-05
• [Presentation] 表面凹凸処理により接触面積を低減した細胞付着防止マニピュレータ (2015)
  • Author(s): 洞出光洋(大阪大学) 小嶋勝(大阪大学) 神山和人(大阪大学)蔵田智之(大阪大学) 前泰志(大阪大学) 新井健生(大阪大学)
  • Organizer: 第33回日本ロボット学会学術講演会
  • Place of Presentation: 東京電機大学東京千住キャンパス, 東京都足立区
  • Year and Date: 2015-09-03 – 2015-09-05
• [Presentation] 2本指マイクロハンド操作のための視触覚融合インタフェース (2015)
  • Author(s): 神山和人（阪大） 小嶋勝（阪大） 洞出光洋（阪大）前 泰志（阪大） 新井健生（阪大）
  • Organizer: 第33回日本ロボット学会学術講演会
  • Place of Presentation: 東京電機大学東京千住キャンパス, 東京都足立区
  • Year and Date: 2015-09-03 – 2015-09-05
• [Presentation] 微小物把持支援のための自動ステージの構築 (2015)
  • Author(s): 山本幸太朗 小嶋勝 洞出光洋 神山和人 前泰志 新井健生(大阪大学)
  • Organizer: 第33回日本ロボット学会学術講演会
  • Place of Presentation: 東京電機大学東京千住キャンパス, 東京都足立区
  • Year and Date: 2015-09-03 – 2015-09-05
• [Presentation] Releasing of Adhered Micro-Objects using Local Stream Generated by High Speed Motion of End Effector (2015)
  • Author(s): 2.Eunhye Kim, Masaru Kojima, Kazuto Kamiyama, Mitsuhiro Horade, Yasushi Mae, and Tatsuo Arai
  • Organizer: 2015 IEEE International Conference on Mechatronics and Automation
  • Place of Presentation: Beijing Friendship Hotel, Beijing, China
  • Year and Date: 2015-08-03 – 2015-08-05
  • Int'l Joint Research
• [Presentation] Generation of Rotational Flow for Formation of Spheroid by Using Microfluidics Device (2015)
  • Author(s): 3.Masaru Kojima, Mitsuhiro Horade, Hirochika Takai, Kenichi Ohara, Tamio Tanikawa, Kazuto Kamiyama, Yasushi Mae and Tatsuo Arai
  • Organizer: 2015 IEEE International Conference on Mechatronics and Automation
  • Place of Presentation: Beijing Friendship Hotel, Beijing, China
  • Year and Date: 2015-08-03 – 2015-08-05
  • Int'l Joint Research
• [Presentation] Development of a Real-time Local Environment Stimulation System with Visual Feedback Control (2015)
  • Author(s): 1.Takahiro Motoyoshi, Masaru Kojima, Kenichi Ohara, Mitsuhiro Horade, Kazuto Kamiyama, Yasushi Mae and Tatsuo Arai
  • Organizer: 2015 IEEE International Conference on Robotics and Automation
  • Place of Presentation: Washington State Convention & Trade Center (WSCC), Seattle, Wachington, USA
  • Year and Date: 2015-05-26 – 2015-05-30
  • Int'l Joint Research
• [Presentation] 表面ナノ凹凸加工付エンドエフェクタを利用した把持対象物のリリース性能の評価 (2015)
  • Author(s): 洞出 光洋,蔵田 智之,小嶋 勝,神山 和人,前 泰志,新井 健生
  • Organizer: ロボティクス・メカトロニクス講演会2015講演論文集
  • Place of Presentation: 京都市勧業館「みやこめっせ」, 京都府京都市
  • Year and Date: 2015-05-17 – 2015-05-19
• [Presentation] 板状エンドエフェクタを用いた細胞剛性計測手法の提案 (2015)
  • Author(s): ニャムドルジハンガイ,小嶋勝,洞出光洋,神山和人,境慎司,前泰志,新井健生
  • Organizer: ロボティクス・メカトロニクス講演会2015講演論文集
  • Place of Presentation: 京都市勧業館「みやこめっせ」, 京都府京都市
  • Year and Date: 2015-05-17 – 2015-05-19
• [Presentation] 二次元任意形状の積層による三次元細胞構造の提案 (2015)
  • Author(s): 高田 賢,小嶋 勝,洞出 光洋,神山 和人,前 泰志,新井 健生
  • Organizer: ロボティクス・メカトロニクス講演会2015講演論文集
  • Place of Presentation: 京都市勧業館「みやこめっせ」, 京都府京都市
  • Year and Date: 2015-05-17 – 2015-05-19
• [Presentation] 細胞・足場間相互作用計測システムの開発 (2015)
  • Author(s): 平野 佑,小嶋 勝,洞出 光洋,神山 和人,前 泰志,新井 健生
  • Organizer: ロボティクス・メカトロニクス講演会2015講演論文集
  • Place of Presentation: 京都市勧業館「みやこめっせ」, 京都府京都市
  • Year and Date: 2015-05-17 – 2015-05-19
• [Presentation] リアルタイム局所化学刺激システムの開発と応用 (2015)
  • Author(s): 小嶋 勝,洞出 光洋,神山 和人,大原賢一,前 泰志,新井 健生
  • Organizer: ロボティクス・メカトロニクス講演会2015講演論文集
  • Place of Presentation: 京都市勧業館「みやこめっせ」, 京都府京都市
  • Year and Date: 2015-05-17 – 2015-05-19
• [Presentation] ヒータアレイデバイスを利用した微小対象物の任意パターニング手法の開発 (2015)
  • Author(s): 洞出 光洋,小嶋 勝,神山 和人,前 泰志,新井 健生
  • Organizer: ロボティクス・メカトロニクス講演会2015講演論文集
  • Place of Presentation: 京都市勧業館「みやこめっせ」, 京都府京都市
  • Year and Date: 2015-05-17 – 2015-05-19
• [Presentation] ハイドロゲルファイバを用いた細胞組織構築システム (2015)
  • Author(s): 福島英、小嶋 勝，大原賢一、洞出 光洋，神山和人、境 慎司、前 泰志， 新井 健生
  • Organizer: ロボティクス・メカトロニクス講演会2015講演論文集
  • Place of Presentation: 京都市勧業館「みやこめっせ」, 京都府京都市
  • Year and Date: 2015-05-17 – 2015-05-19
• [Presentation] エンドエフェクタの振動により誘起される局所流速場の現象解析 (2015)
  • Author(s): 服部 貴享,神山 和人,小嶋 勝,洞出 光洋, 前 泰志,新井 健生
  • Organizer: ロボティクス・メカトロニクス講演会2015講演論文集
  • Place of Presentation: 京都市勧業館「みやこめっせ」, 京都府京都市
  • Year and Date: 2015-05-17 – 2015-05-19
• [Book] Hyper Bio Assembler for 3D Cellular Systems (Chapter 7) (2015)
  • Author(s): Masaru Kojima, Yasushi Mae, Kenichi Ohara, Mitsuhiro Horade, Kazuto Kamiyama and Tatsuo Arai
  • Total Pages: 349 (93-128)
  • Publisher: Springer
• [Remarks] 超高速バイオアセンブラHP
  • URL: http://bio-asm.jp/
• [Patent(Industrial Property Rights)] 表面ナノ凹凸構造を有するマイクロマニピュレーション用エンドエフェクタ、及び表面ナノ凹凸構造の製造方法 (2015)
  • Inventor(s): 洞出光洋，小嶋勝，神山和人，前泰志，新井健生
  • Industrial Property Rights Holder: 洞出光洋，小嶋勝，神山和人，前泰志，新井健生
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願２０１５―０８１４６６
  • Filing Date: 2015-04-13