3D Tissue Construction by using MEMS technology

2015 Fiscal Year Final Research Report

• Project Area: Hyper Bio Assembler for 3D Celluler Innovation
• Project/Area Number: 23106008
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: The University of Tokyo
• Principal Investigator: Shoji Takeuchi 東京大学, 生産技術研究所, 教授 (90343110)
• Co-Investigator(Kenkyū-buntansha): 尾上 弘晃 慶應義塾大学, 理工学部, 講師 (30548681)
• Co-Investigator(Renkei-kenkyūsha): MORIMOTO Yuya 東京大学, 生産技術研究所, 助教 (60739233) ; SHIGETOMI Kaori (KURIBAYASHI Kaori) 北海道大学, 高等教育推進機構, 特任准教授 (90431816) ; TOMOIKE Fumiaki 名古屋大学, 物質科学国際研究センター, 助教 (70708586)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 知能機械 / マイクロマシン / マイクロ・ナノデバイス / 細胞・組織 / 再生医学

Outline of Final Research Achievements

We have developed cell-cultured microfabricated microplates for manipulating adherent cells. We succeeded in three-dimensional manipulation of the adherent cells by handling the microplates with micro-tweezers, fluid flows, magnetic fields and cell traction force. In addition, we have also developed cell-cultured microplates connected with hinges, and constructed three-dimensional cellular structures by folding the microplates by the cell traction force. By embedding permalloy pieces in the cell-cultured microplates, we achieved magnetic handling of the cells on the microplates without physical contacts to both cells and microplates. Using the magnetized microplates, we developed a vertical confocal observation system. This system enables us to observe the boundary of cell membrane at a high-resolution. These progresses will contribute to construction of three-dimensional cellular structures, analysis of cellular interaction, and high-resolution observation of cells.

Free Research Field

バイオMEMS