Fabrication of structually comlicated soft tissue such as liver-tissues and understanding its biological function of teh fabricated liver-tissue

2015 Fiscal Year Final Research Report

• Project Area: Hyper Bio Assembler for 3D Celluler Innovation
• Project/Area Number: 23106009
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: YAMATO MASAYUKI 東京女子医科大学, 医学部, 教授 (40267117)
• Co-Investigator(Kenkyū-buntansha): AKIYAMA Yoshikatsu 東京女子医科大学, 医学部, 講師 (20349640) ; NAKAYAMA Masamichi 東京女子医科大学, 医学部, 講師 (00338980) ; KOBAYASHI Jun 東京女子医科大学, 医学部, 講師 (20385404) ; NAGASE Kenichi 東京女子医科大学, 医学部, 講師 (10439838) ; TAKAHAHI Hironobu 東京女子医科大学, 医学部, 助教 (00710039)
• Co-Investigator(Renkei-kenkyūsha): SHIMIZU Tatsuya 東京女子医科大学, 医学部, 教授 (40318100) ; SHIMIZU Tatsuya 早稲田大学, 理工学術院, 講師 (70468867)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 細胞シート工学 / 肝細胞 / 温度応答性細胞培養表面 / 共培養 / 軟組織

Outline of Final Research Achievements

We have newly developed heparin-immobilized temperature-responsive cell culture surface (TRCS), further modifying the surfaces with growth factor to fabricate hepatic-cells sheet. We have demonstrated that hepatic-cells sheet was readily fabricated with growth-factor and heparin complex immobilized surface, maintaining physiological function characteristic of hepatic-tissue for longer period. Micro-patterned TRCS was also developed, being applied to fabricate nerve-tissue. The surface would be expected as base technology for fabrication of nerve-tissue. A facile method of preparation of TRCS without special equipment and reagents were newly developed. We also innovated robotics to create robot, which attained precise and multiple micro-contacting, and devise to transplant and/or carry cell-sheet easily for short time. Rapid and convenient method for fabricating soft-tissue such as hepatic and nerve tissues would be attained, combing those techniques.

Free Research Field

再生医療、幹細胞生物学