Transport nanodevice immobilized specific biomolecules

2015 Fiscal Year Final Research Report

• Project Area: Molecular Science for Nanomedicine
• Project/Area Number: 23107005
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: The University of Tokyo
• Principal Investigator: ISHIHARA KAZUHIKO 東京大学, 工学(系)研究科(研究院), 教授 (90193341)
• Co-Investigator(Kenkyū-buntansha): INOUE Yuuki 東京大学, 大学院工学系研究科, 助教 (40402789)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: ナノ粒子 / 生体親和性 / 蛍光イメージング / 細胞内輸送

Outline of Final Research Achievements

Polymer nanoparticles as functional in-cell devices were prepared using a cytocompatible phospholipid polymer. We have found that the polymeric nanoparticles embedding quantum dots (QDs) covered with the phospholipid polymers showed resistance to cellular uptake. On the other hand, when an arginine octapeptide (R8), which was one of the cell penetrating peptide, was immobilized at the surface of the nanoparticles, they could penetrate the membrane of HeLa cells effectively. We also investigated the surface functionalization of the nanoparticles and transportation of the nanoparticle into cells. the QDs covered with the pH-responsible phospholipid polymer could be obtained. The pH responsibility reflected inner circumstances of cells. Thus, we could determine the location and local pH circumstances using the QD/phospholipid polymer nanoparticles.　The results provide useful information for delivering the bioactive reagents into cells.

Free Research Field

バイオマテリアル工学