2015 Fiscal Year Final Research Report
Live-cell 3D imaging analysis of the dynamics of epithelial cells and functional molecules in epithelial tubular formation
| Project Area | Regulation of polarity signaling during morphogenesis, remodeling, and breakdown of epithelial tubular structure |
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| Project/Area Number |
23112005
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2016-03-31
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| Keywords | 上皮細胞 / 上皮管腔組織 / アクチン骨格 / RhoGEF / Rho / メカノシグナル / イメージング |
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| Outline of Final Research Achievements |
In this study, we investigated the roles of Rho-GEFs, Farp1 and Solo, which are involved in mechanoresponses, in the ordering and the tubule formation of epithelial cells. We found that Farp1 facilitates the adhesion-dependent growth of mammary epithelial cell through integrins. We found that Solo binds to a simple epithelium-specific intermediate filament, keratin8 and keratin18, and Solo is required to organize and maintain of the keratin8/18 network in epithelial cells. We also showed that Solo is required to activate RhoA and to generate the resistance force in response to tensile-force application. Furthermore, we showed that knockdown of Solo accelerates the velocity of collective cell migration of MDCK cells, and enlarges the lumen area of HGF-induced tubule of 3D-cultured MDCK cells.
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| Free Research Field |
分子細胞生物学
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