異種トゲウオ間のゲノム不適合と生殖隔離の分子機構

2015 Fiscal Year Annual Research Report

• Project Area: Correlative gene system: establishing next-generation genetics
• Project/Area Number: 23113007
• Research Institution: National Institute of Genetics
• Principal Investigator: 北野 潤 国立遺伝学研究所, 集団遺伝研究系, 教授 (80346105)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 種分化 / 生殖隔離 / クロマチン

Outline of Annual Research Achievements

まず、雑種不妊の原因遺伝子に関して、培養細胞系での近縁種間の機能的な際について見いだした。特に、この分子が、SUMOタンパク質が存在するような領域にあることがわかってきた。これまで、近縁種間でのクロマチン制御分子の機能的な差異については、ハエやマウスや植物で報告があるのみであり、実際の野外生物での例としては初めてであると考えられる。現在はこの分子のノックアウトイトヨも解析中であり、来年度にその結果を踏まえて論文発表へと進めて行くことが期待される。また、イトヨの腎臓からとった初代培養細胞を増殖させることにも成功した。この実験システムは、私の研究室において新しい可能性を提供するものであり、非常に貴重に重要な成果と言える。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Whole-genome sequencing reveals small genomic regions of introgression in an introduced crater lake population of threespine stickleback. (2016)
  • Author(s): Yoshida, K., Miyagi, R., Mori, S., Takahashi, A., Makino, T., Toyoda, A., Fujiyama, A., and Kitano, J.
  • Journal Title: Ecology and Evolution Volume: 65 Pages: 2190-2204
  • DOI: 10.1002/ece3.2047
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Comparison of freshwater tolerance during spawning migration between two sympatric Japanese marine threespine stickleback species (2016)
  • Author(s): Ishikawa, A., Kusakabe, M., Kume, M., and Kitano, J.
  • Journal Title: Evolutionary Ecology Research Volume: in press Pages: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Trophic niche differentiation and phenotypic divergence among cryptic species of Japanese ninespine stickleback (2016)
  • Author(s): Ravinet, M., Ishikawa, A., and Kitano, J.
  • Journal Title: Evolutionary Ecology Research Volume: in press Pages: in press
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Toward conservation of genetic and phenotypic diversity in Japanese sticklebacks (2016)
  • Author(s): Kitano, J., Moris, S.
  • Journal Title: Genes and Genetic Systems Volume: in press Pages: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Genetic mechanisms of colonization of new niches in sticklebacks (2015)
  • Author(s): Jun Kitano
  • Organizer: Lecture Series in Ecology and Evolution
  • Place of Presentation: University of Bern, Switzerland
  • Year and Date: 2015-10-11 – 2015-10-11
  • Invited
• [Presentation] Drivers of sex chromosome-autosome fusions and their roles in speciation (2015)
  • Author(s): Jun Kitano
  • Organizer: AGA Presidents Symposium, Chromosome Evolution: Molecular Mechanisms and Evolutionary Consequences
  • Place of Presentation: Bainbridge Island, USA
  • Year and Date: 2015-08-17 – 2015-08-20
  • Int'l Joint Research / Invited
• [Presentation] Why are there so many species with Y- autosome fusions? (2015)
  • Author(s): Kitano J. Pennell M., Kirkpatrick M., Otto S., Vamosi J., Peichel C.
  • Organizer: ESEB 2015
  • Place of Presentation: Lausanne, Switzerland
  • Year and Date: 2015-08-10 – 2015-08-14
  • Int'l Joint Research
• [Presentation] Genome-wide expression QTL (eQTL) analysis reveals trans-acting eQTL hotspots underlying transcriptome divergence between Threespine Stickleback ecotypes. (2015)
  • Author(s): Asano Ishikawa, Makoto Kusakabe, Kohta Yoshida, Takashi Makino, Atsushi Toyoda, Asao Fujiyama, and Jun Kitano
  • Organizer: Stickleback Conference 2015
  • Place of Presentation: New York Stony Brook, USA
  • Year and Date: 2015-07-26 – 2015-07-31
  • Int'l Joint Research