2016 Fiscal Year Final Research Report
Effect of rDNA instability to chromosome and cellular functions
| Project Area | Functions of non-coding DNA region for genome integrity |
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| Project/Area Number |
23114002
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo (2014-2016)
National Institute of Genetics (2011-2013) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
IIDA Tetsushi 東京大学, 分子細胞生物学研究所, 助教 (60391851)
AKAMATSU Yufuko 東京大学, 分子細胞生物学研究所, 助教 (50381661)
SASAKI Mariko 東京大学, 分子細胞生物学研究所, 助教 (50722013)
HORIGOME Chihiro 東京大学, 分子細胞生物学研究所, 助教 (10771206)
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| Research Collaborator |
SAKA Kimiko 国立遺伝学研究所, 技術支援員
HAEIWA Haruna 総合研究大学院大学, 大学院生
UNOZAWA Eri 総合研究大学院大学, 大学院生
TAKAHASHI Akihiro 総合研究大学院大学, 大学院生
WAKATSUKI Tsuyoshi 東京工業大学, 大学院, 大学院生
SUZUKI Yu 東京工業大学, 大学院, 大学院生
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| Project Period (FY) |
2011-04-01 – 2016-03-31
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| Keywords | 非コードDNA / リボソームRNA遺伝子 / 細胞老化 / ゲノムの安定性 / クロマチン / 反復配列 / 複製阻害 / DNAの組換え修復 |
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| Outline of Final Research Achievements |
We identified 708 rDNA unstable mutants. The number corresponds to ~10 % of total genes. In one of the rDNA unstable mutants rtt109, that codes a histone acethylase, we found that recombination repair process of damaged rDNA doesn’t work and rolling circle type replication is induced. By analysis of other rDNA unstable mutants, such as tel1, we found that damaged rDNA was relocated to the nuclear pore from nucleolus. The relocation is thought to be important to prevent improper recombination that induces rDNA instability. In terms of the relationship between rDNA stability and lifespan, we got direct evidence that rDNA instability caused by non-coding transcription rDNA reduces replicative lifespan. We also identified some non-coding functional element and the associating proteins in the rDNA in mammalian cells. They inhibit DNA replication and prevent the collision between DNA replication and rDNA transcription.
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| Free Research Field |
分子遺伝学
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