2016 Fiscal Year Final Research Report
Functional role of non-coding DNA region in DNA damage tolerance
| Project Area | Functions of non-coding DNA region for genome integrity |
|---|
| Project/Area Number |
23114007
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Biological Sciences
|
|---|
| Research Institution | Gakushuin University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011-04-01 – 2016-03-31
|
| Keywords | DNA修復 / DNA相同組換え |
|---|
| Outline of Final Research Achievements |
Genomic integrity is constantly challenged by damage from a variety of sources such as certain chemical agents and UV radiation. The repetitive sequences and chromatin compaction characteristic of non-coding DNA are known to constitute a barrier for DNA replication and repair.Hence, DNA damage and replication stress caused in non-coding DNA regions are implicated in genomic instability. Yeast Srs2, conserved from bacteria to humans, plays important roles in the integrity of repetitive sequences by regulating homologous recombination (HR) pathway. In this study, we demonstrated that Srs2 participates in essential pathways preventing accumulation of inter-homolog recombination intermediates, thereby reducing the risk of genome instability. This work also identified novel H3/H4 alleles involved in replication stress tolerance, which requires a functional HR pathway. Thus, our data identify novel roles of Srs2 and histone H3/H4 in regulating the HR pathway.
|
| Free Research Field |
分子遺伝学
|
