Development of novel methods for single cell quantification with microdevices

2015 Fiscal Year Final Research Report

• Project Area: Spying minority in biological phenomena -Toward bridging dynamics between individual and ensemble processes-
• Project/Area Number: 23115002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: Noji Hiroyuki 東京大学, 工学(系)研究科(研究院), 教授 (00343111)
• Co-Investigator(Kenkyū-buntansha): WATANABE Tomonobu 国立研究開発法人理化学研究所, 生命システム研究センター, チームリーダー (00375205) ; ICHIMURA Taro 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (50600748)
• Co-Investigator(Renkei-kenkyūsha): FUJITA Katsumasa 大阪大学, 工学研究科, 准教授 (80362664)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 1分子生物物理 / マイクロデバイス / 超解像顕微鏡 / 蛍光プローブ

Outline of Final Research Achievements

Noji group in the University of Tokyo aimed to develop novel digital counting quantification methods with single-molecule or single-particle sensitivity by use of femtoliter chamber array device. Digital ELISA with unprecedentedly high sensitivity was established and applied for counting of absolute numbers of protein molecules expressed in single cells. Digital Virus counting method was also developed that revealed the only a few % of total virus population possess the capability of infection. Watanabe group in Riken developed novel imaging methods for protein quantification such as multicolor single-molecule imaging method and fundamental intracellular parameters that represent whole cellular activities such as total protein abundance and gross protein diffusion coefficient.

Free Research Field

1分子生物物理、マイクロデバイス、バイオイメージング