Exploring the mechanisms of metabolic control of gene expression through new approaches of nutrigenomics

2015 Fiscal Year Final Research Report

• Project Area: Crosstalk of transcriptional control and energy pathways by hub metabolites
• Project/Area Number: 23116006
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Tsukuba
• Principal Investigator: Yahagi Naoya 筑波大学, 医学医療系, 准教授 (60420246)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: エネルギー代謝 / 栄養 / ゲノム / 転写

Outline of Final Research Achievements

The principal investigator has established a quantitative system that combines adenovirus-mediated transduction of luciferase reporter gene into the liver with in vivo imaging system (IVIS) technique (in vivo Ad-luc analytical system) to create an intra-organ assay system for the analysis of energy metabolism and metabolic signals. Moreover, we have developed the Transcription Factor Expression Library (TFEL), which covers all known transcription factors encoded by the mouse genome, and created a method for the analysis of transcription complexes using this library (TFEL scan method). We employed these methods to identify the genomic regions acted upon by a range of signals related to energy metabolism and elucidated the mechanisms underlying the metabolic control of gene expression.

Free Research Field

代謝学、栄養学、分子生物学