Cross-regulation of the maintenance and mutagenesis of genomic sequence and epigenetic information

2015 Fiscal Year Final Research Report

• Project Area: Crosstalk of transcriptional control and energy pathways by hub metabolites
• Project/Area Number: 23116008
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kobe University
• Principal Investigator: Sugasawa Kaoru 神戸大学, バイオシグナル研究センター, 教授 (70202124)
• Co-Investigator(Renkei-kenkyūsha): SAKAI Wataru 神戸大学, バイオシグナル研究センター, 助教 (70526251) ; IWAI Shigenori 大阪大学, 大学院基礎工学研究科, 教授 (10168544)
• Research Collaborator: INASE Aki ; MATSUMOTO Syota ; TONE Daisuke ; NAKAMURA Tomohumi ; NAKANISHI Seiya ; KAKUMU Erina ; KOYAMA Saki ; Kishimoto Aiko ; UEMURA Mika ; KANEKO Yuki ; GOTO Motonari
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 遺伝子 / エピゲノム / 発現制御 / DNA損傷修復 / 変異

Outline of Final Research Achievements

In this study, it is suggested that formation of heterochromatin-like structures is involved in regulation of intracellular localization of DNA damage recognition factors for nucleotide excision repair. We also elucidate novel roles of various post-translational modifications (ubiquitination, SUMOylation, acetylation, etc.) in functional regulation of the DDB2 protein, which is involved in crosstalks between DNA repair and transcription, thereby regulating cellular DNA damage responses. Furthermore, the molecular mechanism is uncovered for regulating stability of thymine DNA glycosylase (TDG), which is a key factor involved in both base excision repair suppressing spontaneous mutagenesis as well as active DNA demethylation. TDG is also shown to interact functionally with the nucleotide excision repair pathway by affecting functions of the xeroderma pigmentosum group C (XPC) protein.

Free Research Field

細胞生物学