共生非依存的に進化したオルガネラによるマトリョーシカ化機構

2015 Fiscal Year Annual Research Report

• Project Area: "Matryoshka"-type evolution of eukaryotes
• Project/Area Number: 23117008
• Research Institution: Nagasaki University
• Principal Investigator: 金子 修 長崎大学, 熱帯医学研究所, 教授 (50325370)
• Co-Investigator(Kenkyū-buntansha): 坪井 敬文 愛媛大学, プロテオサイエンスセンター, 教授 (00188616)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 原虫 / マラリア / 赤血球 / オルガネラ / 細胞内輸送

Outline of Annual Research Achievements

二次共生由来オルガネラという「オルガネラ二重構造」を内部に持つアピコンプレクス門原虫は、さらに真核細胞へ侵入・改変することでマトリョーシカ構造を発展させる。本研究では赤血球に侵入・改変して発育するマラリア原虫について、種々の分泌オルガネラに局在するタンパク質の網羅的同定と各オルガネラへの輸送に関わる分子の同定を通じて、細胞侵入と寄生成立を可能とする特殊化された分泌オルガネラの機能と起源を明らかにし、マラリア原虫が赤血球を隷属化するマトリョーシカ化発展機構の成立と分子機序を包括的に理解することを目的とする。
平成27年度も赤血球侵入型原虫のオルガネラに局在すると予想されるマラリア分子群に対して作製した抗体により、オルガネラへの局在と機能の解析を継続した。その結果、熱帯熱マラリア原虫メロゾイトの先端部に局在する新規赤血球結合分子としてPfMAS170を同定した。特異抗体が赤血球への侵入を阻害することから、赤血球侵入に重要な役割を果たしていると考えられる。また、感染赤血球内に輸送される原虫分子SURFINの組換え体を含む複合体を精製してプロテオーム解析を行った結果、SURFINとは異なる分子を輸送するのに利用されているトランスロコンの構成分子が検出され、SURFINも本トランスロコンを利用して輸送されることが示唆された。また、従来、輸送に関与することが知られていなかった複数の分子も検出され、それぞれの特異抗体により、組換えSURFINとの結合が確認された。感染赤血球表面に組換えタンパク質が露出しているかどうかを検証するアッセイ系を用いて、SURFINの細胞内領域のドメインが、赤血球膜直下の膜構造物から膜表面への露出に必要であることを明らかにした。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Weill Cornell Medical School/NIAID, NIH/Case Western Reserve University(米国)
  • Country Name: U.S.A.
  • Counterpart Institution: Weill Cornell Medical School/NIAID, NIH/Case Western Reserve University
  • # of Other Institutions: 3
• [Int'l Joint Research] Chulalongkorn University/Mahidol University/AFRIMS(タイ)
  • Country Name: THAILAND
  • Counterpart Institution: Chulalongkorn University/Mahidol University/AFRIMS
  • # of Other Institutions: 1
• [Int'l Joint Research] Oxford University(英国)
  • Country Name: UNITED KINGDOM
  • Counterpart Institution: Oxford University
• [Int'l Joint Research] Kangwon National University(韓国)
  • Country Name: KOREA (REP. OF KOREA)
  • Counterpart Institution: Kangwon National University
• [Int'l Joint Research] Karolinska Institutet(スウェーデン)
  • Country Name: SWEDEN
  • Counterpart Institution: Karolinska Institutet
• [Int'l Joint Research]
  • # of Other Countries: 5
• [Journal Article] Ca2+ monitoring in Plasmodium falciparumusing the yellow cameleon-Nano biosensor (2016)
  • Author(s): Pandey K, Ferreira PE, Ishikawa T, Nagai T, Kaneko O, Yahata K
  • Journal Title: Sci Rep Volume: 6 Pages: 23454-23454
  • DOI: 10.1038/srep23454
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ungulate malaria parasites (2016)
  • Author(s): Templeton TJ, Asada M, Jiratanh M, Ishikawa SA, Tiawsirisup S, Sivakumar T, Namangala B, Takeda M, Mohkaew K, Ngamjituea S, Inoue N, Sugimoto C, Inagaki Y, Suzuki Y, Yokoyama N, Kaewthamasorn M, Kaneko O
  • Journal Title: Sci Rep Volume: 6 Pages: 23230-23230
  • DOI: 10.1038/srep23230
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Three-dimensional analysis of morphological changes in the malaria parasite infected red blood cell by serial block-face scanning electron microscopy (2016)
  • Author(s): Sakaguchi M, Miyazaki N, Fujioka H, Kaneko O, Murata K
  • Journal Title: J Struct Biol Volume: 193 Pages: 162-171
  • DOI: 10.1016/j.jsb.2016.01.003
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Establishment of transient and stable transfection systems for Babesia ovata (2016)
  • Author(s): Hakimi H, Yamagishi J, Kegawa Y, Kaneko O, Kawazu SI, Asada M
  • Journal Title: Parasit Vectors Volume: 9 Pages: 171-171
  • DOI: 10.1186/s13071-016-1439-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Isolation of invasive Plasmodium yoelii merozoites with a long half-life to evaluate invasion dynamics and potential invasion inhibitors (2015)
  • Author(s): Mutungi JK, Yahata K, Sakaguchi M, Kaneko O
  • Journal Title: Mol Biochem Parasitol Volume: 204 Pages: 26-33
  • DOI: 10.1016/j.molbiopara.2015.12.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The cytoplasmic region of Plasmodium falciparumSURFIN4.2 is required for transport from Maurer's clefts to the red blood cell surface (2015)
  • Author(s): Kagaya W, Miyazaki S, Yahata K, Ohta N, Kaneko O
  • Journal Title: Trop Med Health Volume: 43 Pages: 265-272
  • DOI: 10.2149/tmh.2015-38
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transfection ofBabesia bovis by double selection with WR99210 and Blasticidin-S and its application for functional analysis of thioredoxin peroxidase-1 (2015)
  • Author(s): Asada M, Yahata K, Hakimi H, Yokoyama N, Igarashi I, Kaneko O, Suarez CE, Kawazu S
  • Journal Title: PLoS One Volume: 10 Pages: e0125993
  • DOI: 10.1371/journal.pone.0125993
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rodent malaria lactate dehydrogenase assay provides a high throughput solution for in vivo vaccine studies (2015)
  • Author(s): Otsuki H, Yokouchi Y, Iyoku N, Tachibana M, Tsuboi T, Torii M
  • Journal Title: Parasitol Int Volume: 64 Pages: 60-63
  • DOI: 10.1016/j.parint.2015.02.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Plasmodium falciparum: Genetic diversity and complexity of infections in an isolated village in western Thailand (2015)
  • Author(s): Tanabe K, Zollner G, Vaughand JA, Sattabongkot J, Khuntirat B, Honma H, Mita T, Tsuboi T, Coleman R
  • Journal Title: Parasitol Int Volume: 64 Pages: 260-266
  • DOI: 10.1016/j.parint.2013.09.011
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Immunoprofiling of the tryptophan-rich antigen family in Plasmodium vivax (2015)
  • Author(s): Wang B, Lu F, Cheng Y, Chen JH, Jeon HY, Ha KS, Cao J, Nyunt MH, Han JH, Lee SK, Kyaw MP, Sattabongkot J, Takashima E, Tsuboi T, Han ET
  • Journal Title: Infect Immun Volume: 83 Pages: 3083-3095
  • DOI: 10.1128/IAI.03067-14
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Discovery of novel Plasmodium falciparum pre-erythrocytic antigens for vaccine development (2015)
  • Author(s): Aguiar JC, Bolton J, Wanga J, Sacci JB, Iriko H, Mazeika JK, Han ET, Limbach K, Patterson NB, Sedegah M, Cruz AM, Tsuboi T, Hoffman SL, Carucci D, Hollingdale MR, Villasante ED, Richie TL
  • Journal Title: PLoS One Volume: 10 Pages: e0136109
  • DOI: 10.1371/journal.pone.0136109
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparative assessment of transmission-blocking vaccine candidates against Plasmodium falciparum (2015)
  • Author(s): Kapulu MC, Da DF, Miura K, Li Y, Blagborough AM, Churcher TS, Nikolaeva D, Williams AR, Goodman AL, Sangare I, Turner AV, Cottingham MG, Nicosia A, Straschil U, Tsuboi T, Gilbert SC, Long CA, Sinden RE, Draper SJ, Hill AVS, Cohuet A, Biswas S
  • Journal Title: Sci Rep Volume: 5 Pages: 11193-11193
  • DOI: 10.1038/srep11193
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic diversity of transmission-blocking vaccine candidate Pvs48/45 in Plasmodium vivax populations in China. (2015)
  • Author(s): Feng H, Gupta B, Wang M, Zheng W, Zheng L, Zhu X, Yang Y, Fang Q, Luo E, Fan Q, Tsuboi T, Cao Y, Cui L
  • Journal Title: Parasit Vectors Volume: 8 Pages: 615-615
  • DOI: 10.1186/s13071-015-1232-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] マラリア原虫の膜タンパク質輸送マシーナリーの解析 (2016)
  • Author(s): 宮崎真也, 加賀谷渉, Zhu X, Chitama B, 矢幡一英, 金子修
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-03-26 – 2016-03-29
• [Presentation] 熱帯熱マラリア原虫SURFIN4.2の細胞内領域はマウレル裂から赤血球表面への輸送に必要である (2016)
  • Author(s): 加賀谷渉、宮崎真也、矢幡一英、太田伸生、金子修
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）
  • Year and Date: 2016-03-19 – 2016-03-20
• [Presentation] ネズミマラリア原虫赤血球侵入リガンドEBL領域4 の機能解析 (2016)
  • Author(s): 大槻均，菅井千明，近藤陽子，入子英幸，石野智子，金子修，坪井敬文，鳥居本美
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）
  • Year and Date: 2016-03-19 – 2016-03-20
• [Presentation] Tet-Onによる遺伝子コンディショナルノックアウト法の開発 (2016)
  • Author(s): 麻田正仁、外川裕人、矢幡一英、金子修
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）
  • Year and Date: 2016-03-19 – 2016-03-20
• [Presentation] Establishment of a stable transfection system for Babesia ovata and its application for functional gene studies (2016)
  • Author(s): Hakimi H, Yamagishi J, Umemiya-Shirafuji R, Kegawa Y, Kaneko O, Kawazu S-I, Asada M
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）
  • Year and Date: 2016-03-19 – 2016-03-20
• [Presentation] マラリア原虫のRab5b GTPaseは寄生胞膜の感染赤血球側に運ばれる (2016)
  • Author(s): 海老根一生，平井智浩, 平井誠，坂口美亜子，矢幡一英，金子修，野崎智義, 中野由美子
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）
  • Year and Date: 2016-03-19 – 2016-03-20
• [Presentation] Plasmodium yoelii遺伝子を標的としたテトラサイクリン発現誘導システムの確立 (2016)
  • Author(s): 外川裕人、麻田正仁、矢幡一英、金子修
  • Organizer: 85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）
  • Year and Date: 2016-03-19 – 2016-03-20
• [Presentation] Isolation method of invasive Plasmodium yoelii merozoites with a long half-life to evaluate erythrocyte invasion dynamics and potential invasion inhibitors (2016)
  • Author(s): Mutungi JK, Yahata K, Sakaguchi M, Kaneko O
  • Organizer: Molecular Approach to Malaria 2016
  • Place of Presentation: Lorne (Austraria)
  • Year and Date: 2016-02-21 – 2016-02-25
  • Int'l Joint Research
• [Presentation] The tryptophan-rich domain of Plasmodium falciparum SURFIN4.2 is required for its transport from Maurer’s clefts to the surface of red blood cell (2016)
  • Author(s): Miyazaki S, Kagaya W, Yahata K, Ohta N, Kaneko O
  • Organizer: Molecular Approach to Malaria 2016
  • Place of Presentation: Lorne (Austraria)
  • Year and Date: 2016-02-21 – 2016-02-25
  • Int'l Joint Research
• [Presentation] Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor (2016)
  • Author(s): Yahata K, Pandey K, Ferreira PE, Ishikawa T, Kaneko O
  • Organizer: Molecular Approach to Malaria 2016
  • Place of Presentation: Lorne (Austraria)
  • Year and Date: 2016-02-21 – 2016-02-25
  • Int'l Joint Research
• [Presentation] 誘電泳動現象を用いた赤血球の変形能計測デバイス (2016)
  • Author(s): 額賀正行、矢口友幸、金秀炫、矢幡一英、藤井輝夫、金子修、木村啓志
  • Organizer: シンポジウム：細胞アッセイ技術の現状と将来
  • Place of Presentation: 東京大学生産技術研究所（東京都目黒区）
  • Year and Date: 2016-01-19
• [Presentation] Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor (2016)
  • Author(s): Pandey K, Ferreira PE, Ishikawa T, Kaneko O, Yahata K
  • Organizer: U.S.-Japan Cooperative Medical Sciences Program (USJCMSP)
  • Place of Presentation: Bethesda (USA)
  • Year and Date: 2016-01-13 – 2016-01-14
  • Int'l Joint Research / Invited
• [Presentation] WGCFS: an innovative technology expressing quality proteins for the post-genome malaria vaccine candidate discovery (2016)
  • Author(s): Tsuboi T
  • Organizer: U.S.-Japan Cooperative Medical Sciences Program (USJCMSP)
  • Place of Presentation: Bethesda (USA)
  • Year and Date: 2016-01-13 – 2016-01-14
  • Int'l Joint Research
• [Presentation] 誘電泳動現象を活用した赤血球変形能計測デバイスの開発 (2016)
  • Author(s): 額賀正行、矢口友幸、Kim SooHyeon、矢幡一英、藤井輝夫、金子修、木村啓志
  • Organizer: 日本機械学会 第28回バイオエンジニアリング講演会
  • Place of Presentation: 東京工業大学・大岡山キャンパス（東京都目黒区）
  • Year and Date: 2016-01-09 – 2016-01-10
• [Presentation] 熱帯熱マラリア原虫SURFIN4.2のトリプトファン豊富領域はマウレル裂から赤血球表面への輸送に必要である (2015)
  • Author(s): 加賀谷渉、宮崎真也、矢幡一英、太田伸生、金子修
  • Organizer: 第56回日本熱帯医学会大会
  • Place of Presentation: 大阪大学コンベンションセンター（大阪府吹田市）
  • Year and Date: 2015-12-04 – 2015-12-06
• [Presentation] マラリア原虫による寄生赤血球の改変 (2015)
  • Author(s): Lucky, A、坂口美亜子、宮崎真也、加賀谷渉、片貝祐子、川合覚、矢幡一英、Templeton T、金子修
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会 Workshop
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01 – 2015-12-04
• [Presentation] ポストゲノムマラリアワクチン研究は宿主寄生体関係の総体的理解につながる (2015)
  • Author(s): 坪井敬文
  • Organizer: 第88回日本生化学会大会・第38回日本分子生物学会年会 合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01 – 2015-12-04
  • Invited
• [Presentation] マラリア原虫による感染赤血球へのタンパク質輸送 (2015)
  • Author(s): 宮崎真也, 加賀谷渉, Zhu X, Chitama B, 矢幡一英, 金子修
  • Organizer: 第9回細菌学 若手コロッセウム
  • Place of Presentation: レインボー桜島（鹿児島県鹿児島市）
  • Year and Date: 2015-11-23 – 2015-11-25
• [Presentation] On-chip deformability measurement of malaria parasite-infected red blood cells by dielectrophoretic force (2015)
  • Author(s): Nukaga M, Kim SH, Yahata K, Fujii T, Kaneko O, Kimura H
  • Organizer: μTAS 2015 - The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: Gyeongiu (Korea)
  • Year and Date: 2015-10-25 – 2015-10-29
  • Int'l Joint Research
• [Presentation] Identification of a strain transcending blood-stage malaria vaccine candidate against Plasmodium falciparum among four novel blood-stage antigens (2015)
  • Author(s): Ntege EH, Arisue N, Ito D, Hasegawa T, Palacpac NMQ, Horii T, Takashima E, Tsuboi T
  • Organizer: ASTMH 64th annual meeting
  • Place of Presentation: Philadephia (USA)
  • Year and Date: 2015-10-25 – 2015-10-29
  • Int'l Joint Research
• [Presentation] Isolation method of invasive Plasmodium yoelii merozoites with a long half-life to evaluate erythrocyte invasion dynamics and potential invasion inhibitors (2015)
  • Author(s): Mutungi JK, Yahata K, Sakaguchi M, Kaneko O
  • Organizer: 2nd International Symposium & 4th Annual Researche Meeting on Matryoshka-Type Evolution of Eukaryotic Cells
  • Place of Presentation: University Hall of University of Tsukuba (Tsukuba, Ibaraki)
  • Year and Date: 2015-09-30 – 2015-10-02
  • Int'l Joint Research
• [Presentation] Identification of the proteins interacting with Plasmodium falciparum SURFIN that is exported to the parasite-infected red blood cell (2015)
  • Author(s): Miyazaki S, Kagaya W, Zhu X, Chitama B, Yahata K, Kaneko O
  • Organizer: 2nd International Symposium & 4th Annual Researche Meeting on Matryoshka-Type Evolution of Eukaryotic Cells
  • Place of Presentation: University Hall of University of Tsukuba (Tsukuba, Ibaraki)
  • Year and Date: 2015-09-30 – 2015-10-02
  • Int'l Joint Research
• [Presentation] Proteins in dense granules of malaria merozoite are responsible for the modification of parasitophorous vacuole essential for the parasitism (2015)
  • Author(s): Morita M, Takashima E, Ito D, Iimura T, Torii M, Tsuboi T
  • Organizer: 2nd International Symposium & 4th Annual Research Meeting on Matryoshka-type Evolution of Eukaryotic Cells
  • Place of Presentation: University Hall of University of Tsukuba (Tsukuba, Ibaraki)
  • Year and Date: 2015-09-30 – 2015-10-02
  • Int'l Joint Research
• [Presentation] Coevolution of cellular metabolic systems and mitochondria carrier-type membrane transporters (2015)
  • Author(s): Tozawa Y, Nozawa A, Tsuboi T
  • Organizer: 2nd International Symposium & 4th Annual Research Meeting on Matryoshka-type Evolution of Eukaryotic Cells
  • Place of Presentation: University Hall of University of Tsukuba (Tsukuba, Ibaraki)
  • Year and Date: 2015-09-30 – 2015-10-02
  • Int'l Joint Research
• [Presentation] Functional analyses of sporozoite rhoptry proteins of Plasmodium berghei during target cell invasion (2015)
  • Author(s): Ishino T, Sugino Y, Nozaki M, Murata E, Tokunaga N, Tachibana M, Tsuboi T, Torii M
  • Organizer: 2nd International Symposium & 4th Annual Research Meeting on Matryoshka-type Evolution of Eukaryotic Cells
  • Place of Presentation: University Hall of University of Tsukuba (Tsukuba, Ibaraki)
  • Year and Date: 2015-09-30 – 2015-10-02
  • Int'l Joint Research
• [Presentation] Ca2+ monitoring in Plasmodium falciparum cytosol through yellow cameleon-Nano biosensors (2015)
  • Author(s): Yahata K, Pandey K, Ferreira PE, Ishikawa T, Kaneko O
  • Organizer: Protein island Matsuyama International Symposium
  • Place of Presentation: 松山市男女共同参画推進センター（愛媛県松山市）
  • Year and Date: 2015-09-24 – 2015-09-25
  • Int'l Joint Research / Invited
• [Presentation] Transport and localisation of Plasmodium knowlesi Skeletal Binding Protein 1 (SBP1) in infected monkey and human erythrocytes (2015)
  • Author(s): Lucky AB, Sakaguchi M, Katakai Y, Kawai S, Yahata K, Templeton T, Kaneko O
  • Organizer: The 14th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Yumebutai International Conference Center (Awaji, Hyogo)
  • Year and Date: 2015-09-08 – 2015-09-11
  • Int'l Joint Research
• [Presentation] Transfection of Babesia bovis by Double Selection with WR99210 and Blasticidin-S and Its Application for Functional Analysis of Thioredoxin Peroxidase-1 (2015)
  • Author(s): Asada M, Yahata K, Hakimi H, Yokoyama N, Igarashi I, Kaneko O, Suarez CE, Kawazu S-I
  • Organizer: The 14th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Yumebutai International Conference Center (Awaji, Hyogo)
  • Year and Date: 2015-09-08 – 2015-09-11
  • Int'l Joint Research
• [Presentation] Calcium monitoring in Plasmodium falciparum through Yellow Cameleon-Nano Biosensors (2015)
  • Author(s): Yahata K, Pandey K, Ferreira PE, Ishikawa T, Kaneko O
  • Organizer: The 14th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Yumebutai International Conference Center (Awaji, Hyogo)
  • Year and Date: 2015-09-08 – 2015-09-11
  • Int'l Joint Research
• [Presentation] Establishment of transient and stable transfection system for Babesia ovata (2015)
  • Author(s): Hakimi H, 山岸潤也、外川裕人、田中 健、金子 修、河津信一郎、麻田正仁
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部（青森県十和田市）
  • Year and Date: 2015-09-07 – 2015-09-09
• [Presentation] Development of Tet-On-based conditional gene knockout system for rodent malaria parasite Plasmodium yoelii (2015)
  • Author(s): Asada M, Kegawa Y, Yahata K, Kaneko O
  • Organizer: 第4回感染症若手フォーラム
  • Place of Presentation: アテーナ海月（兵庫県淡路市）
  • Year and Date: 2015-09-06 – 2015-09-08
• [Presentation] Transport and localisation of Plasmodium knowlesi Skeletal Binding Protein 1 (SBP1) in infected monkey and human erythrocytes (2015)
  • Author(s): Lucky AB, Sakaguchi M, Katakai Y, Kawai S, Yahata K, Templeton T, Kaneko O
  • Organizer: 第4回感染症若手フォーラム
  • Place of Presentation: アテーナ海月（兵庫県淡路市）
  • Year and Date: 2015-09-06 – 2015-09-08
• [Presentation] Characterization of Plasmodium vivax subtelomeric transmembrane protein (2015)
  • Author(s): Sungkapong T, Kaewthamasorn, Yahata K, Chotivanich K, Kaneko O
  • Organizer: 第4回感染症若手フォーラム
  • Place of Presentation: アテーナ海月（兵庫県淡路市）
  • Year and Date: 2015-09-06 – 2015-09-08
• [Presentation] 感染赤血球へのタンパク質輸送に必須なマラリア原虫分子の探索 (2015)
  • Author(s): 宮崎真也, 加賀谷渉, Zhu X, Chitama B, 矢幡一英, 金子修
  • Organizer: 第23回分子寄生虫学ワークショップ/第13回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2015-08-30 – 2015-09-02
• [Presentation] 熱帯熱マラリア原虫SURFIN4.2 のマウレル裂から赤血球表面への輸送機構 (2015)
  • Author(s): 加賀谷渉, 宮崎真也, 矢幡一英, 太田伸生, 金子修
  • Organizer: 第23回分子寄生虫学ワークショップ/第13回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2015-08-30 – 2015-09-02
• [Presentation] Plasmodium yoelii 赤血球内侵入型原虫を標的としたテトラサイクリン発現誘導システムの確立 (2015)
  • Author(s): 外川裕人, 麻田正仁, 矢幡一英, 金子修
  • Organizer: 第23回分子寄生虫学ワークショップ/第13回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2015-08-30 – 2015-09-02
• [Presentation] バベシア原虫メロゾイトの宿主赤血球遊出機構 (2015)
  • Author(s): 麻田正仁, Mossaad E, Hakimi H, 矢幡一英, 河津信一郎, 金子修
  • Organizer: 第23回分子寄生虫学ワークショップ/第13回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2015-08-30 – 2015-09-02
• [Presentation] 熱帯熱マラリア原虫の輸送タンパク質 (2015)
  • Author(s): 宮崎 真也 ，加賀谷渉 ，Xiaotong Zhu，矢幡一英 ，金子修
  • Organizer: 第67日本細胞生物学会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2015-06-30 – 2015-07-02
• [Presentation] Pre-erythrocytic vaccines for malaria elimination (2015)
  • Author(s): Tsuboi T
  • Organizer: Malaria R&D in a Time of Global Partnerships
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-06-26 – 2015-06-26
  • Int'l Joint Research / Invited
• [Presentation] Evaluation of Ca2+ Oscillation in Plasmodium falciparum through Yellow Cameleon-Nano Biosensors (2015)
  • Author(s): Kaneko O
  • Organizer: The 12th NUS-Nagasaki Joint Symposium
  • Place of Presentation: Singapore (Singapore)
  • Year and Date: 2015-06-11 – 2015-06-12
  • Int'l Joint Research / Invited
• [Presentation] An integrated approach to tackling malaria in Uganda with special reference to novel malaria vaccine candidate discovery (2015)
  • Author(s): Tsuboi T, Kanoi BN, Egwang TG, Horii T
  • Organizer: E-JUST 2nd International Conference on Innovative Engineering
  • Place of Presentation: Alexandria (Egypt)
  • Year and Date: 2015-05-19 – 2015-05-21
  • Int'l Joint Research / Invited
• [Presentation] Genome-wide immunoscreening of antibodies responsible for Plasmodium falciparum growth inhibitory activity of Malian adult IgG (2015)
  • Author(s): Morita M, Takashima E, Kanoi BN, Miura K, Long CA, Tsuboi T
  • Organizer: 25th Annual Molecular Parasitology/Vector Biology Symposium
  • Place of Presentation: Athens (USA)
  • Year and Date: 2015-04-28 – 2015-04-29
  • Int'l Joint Research
• [Presentation] Identification of Plasmodium falciparum PfMAS170 as novel blood-stage malaria vaccine candidate (2015)
  • Author(s): Takashima E, Sasaoka C, Sakamoto H, Ito D, Takeo S, Sattabongkot J, Tsuboi T
  • Organizer: 25th Annual Molecular Parasitology/Vector Biology Symposium
  • Place of Presentation: Athens (USA)
  • Year and Date: 2015-04-28 – 2015-04-29
  • Int'l Joint Research
• [Presentation] WGCFS: an innovative technology for post-genome malaria vaccine research (2015)
  • Author(s): Tsuboi T
  • Organizer: 25th Annual Molecular Parasitology/Vector Biology Symposium
  • Place of Presentation: Athens (USA)
  • Year and Date: 2015-04-28 – 2015-04-29
  • Int'l Joint Research / Invited