Construction of synthetic genetic circuit for control of cell response

2015 Fiscal Year Final Research Report

• Project Area: Synthetic biology for the comprehension of biomolecular networks
• Project/Area Number: 23119002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Kyushu University
• Principal Investigator: Taizo Hanai 九州大学, (連合)農学研究科(研究院), 准教授 (60283397)
• Co-Investigator(Kenkyū-buntansha): AIBA Hirohumi 名古屋大学, 創薬科学研究科, 教授 (60211687) ; HAMADA Hiroyuki 九州大学, 大学院農学研究院, 助教 (80346840) ; Okamoto Masahiro 九州大学, 大学院農学研究院, 教授 (40211122)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 合成生物学 / シミュレーション / トグルスイッチ / 人工遺伝子回路 / 合成代謝経路 / 2成分制御系 / クオラムセンシング

Outline of Final Research Achievements

The goal of this research project is to construct the synthetic genetic circuit which realizes a desired dynamic behavior. This circuit is composed of 10 or less biological molecular parts. In order to reach this goal, we accomplished 1) construction of genetic toggle switch operating with high stability, 2) application of genetic toggle switch, 3) modification of sensor proteins, 4) reconstruction and modification of quorum sensing system. We used not only molecular biology but also mathematical analysis for the system design of the synthetic genetic circuit. Through these researches, at last, we succeeded to construct “target product producing microorganism autonomously controlled” which could sense cell number and change intracellular metabolite distribution suitable for from cell growth to production according to cell number and produce 3 times more target product than the control strain.

Free Research Field

合成生物学